MATRIX-ASSISTED LASER DESORPTION OF PEPTIDES IN TRANSMISSION GEOMETRY

被引：34

作者：

VERTES, A

BALAZS, L

GIJBELS, R

机构：

[1] Department of Chemistry, University of Antwerp (UIA), Wilrijk, B-2610

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 07期

关键词：

D O I：

10.1002/rcm.1290040709

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The possibility of performing matrix‐assisted laser desorption experiments in transmission geometry is demonstrated for two neuropeptides (substance P and bombesin), for six analogs of the melanocyte‐stimulating hormone (MSH) core and for coliagenase enzyme substrates. Positive‐ and negative‐ion spectra of several peptides are produced without the presence of a metallic substrate. Cationized quasi‐molecular ions are abundant in the positive spectra. Peak broadening in the high‐mass range can be the consequence of overlapping molecular and adduct ions. The presence of synthesis by‐products can be identified readily from the spectra. Ultimately, picogram detection limits are possible for important bioactive peptides and other large molecules. Because of the clearly demonstrated matrix‐assisted laser ionization in a homogeneous environment, metal substrate participation in the volatilization mechanism seems less likely. Copyright © 1990 John Wiley & Sons Limited

引用

页码：263 / 266

页数：4

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