COMPARISON OF A NA+/D-GLUCOSE COTRANSPORTER FROM RAT INTESTINE EXPRESSED IN OOCYTES OF XENOPUS-LAEVIS WITH THE ENDOGENOUS COTRANSPORTER

Epithelial Na+/D-glucose contransport was incorporated into the plasma membrane of Xenopus oocytes after microinjection of poly(A)+-mRNA from rat intestine tissue and was detected by measurements of uptake of [C-14]AMG (methyl alpha-D-glucopyranoside). In mRNA-injected oocytes, the rate of AMG uptake exceeds the rate of endogenous Na+/AMG cotransport by a factor of up to 30. It is demonstrated that the additionally expressed transport differs qualitatively from the endogenous transport with respect to several parameters which is a prerequisite for the demonstration of expression of a foreign transporter: (1) The expressed system is more sensitive to external glucose or AMG and to the specific inhibitor phlorizin, (2) it is less sensitive to external Na+ and to changes in membrane potential, and (3) it is susceptible to inhibition by monoclonal antibodies, known to bind specifically to Na+/glucose contransporters and to modulate the contransport in kidney and intestine. The use of the antibodies allows one to distinguish between endogenous Na+/AMG contransport and foreign cotransport expressed by injection of foreign mRNA. The expression of the foreign transport leads to transport rates that are high enough to detect the electrical current generated by the Na+/glucose contransport. This allows future characterization of the cotransport system under voltage-clamp conditions by analyzing membrane current.