EVIDENCE FOR THE TETRAPLEX STRUCTURE OF THE D(GT)N REPETITIVE SEQUENCES IN SOLUTION

被引:21
作者
BORISOVA, OF
SHCHYOLKINA, AK
TIMOFEEV, EN
FLORENTIEV, VL
机构
[1] V.A. Engelhardt Institute of Molecular Biology, the Russia Academy of Sciences
关键词
TETRAPLEX; PARALLEL STRANDED DNA; D(GT)N SEQUENCE;
D O I
10.1016/0014-5793(92)80985-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ability of oligonucleotides 3'-d(GT)5pO(CH2)6Opd(GT)5-5' (anti[d(GT)]) and 3'-d(GT)5pO(CH2)6Opd(GT)5-3'(par[d(GT)]) to form tertiary structures has been studied. Circular dichroism (CD) as well as the fluorescence of the ethidium bromide (EtBr) complexes with oligonucleotides and hydrodynamic volume measurements in solutions containing 0.01 M phosphate buffer, pH 7 and NaCl in concentrations from 0.1 M to 1 M, have been used. The data obtained in the temperature interval from 3-degrees-C to 10-degrees-C are in good agreement with the structure suggested earlier [1] where the par[d(GT)] and anti[d(GT)] form structures with four parallel strands in which layers of four G-residues alternate with unpaired bulged-out T-residues. Ethidium bromide interacts with the structure in a cooperative manner. Two ethidium bromide molecules intercalate between two layers of four G-residues.
引用
收藏
页码:140 / 142
页数:3
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