CHEMICAL MODIFICATION OF RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR BY POLYETHYLENE-GLYCOL INCREASES ITS BIOLOGICAL-ACTIVITY INVIVO

被引：28

作者：

SATAKEISHIKAWA, R ^{[1
]}

ISHIKAWA, M ^{[1
]}

OKADA, Y ^{[1
]}

KAKITANI, M ^{[1
]}

KAWAGISHI, M ^{[1
]}

MATSUKI, S ^{[1
]}

ASANO, K ^{[1
]}

机构：

[1] KIRIN BREWERY CO LTD, PHARMACEUT LAB, 1-2-2 SOUJA MACHI, MAEBASHI, GUNMA 371, JAPAN

来源：

CELL STRUCTURE AND FUNCTION | 1992年 / 17卷 / 03期

关键词：

GRANULOCYTE-COLONY STIMULATING FACTOR; POLYETHYLENE GLYCOL; CHEMICAL MODIFICATION;

D O I：

10.1247/csf.17.157

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recombinant human granulocyte colony-stimulating factor (rHuG-CSF) produced in Escherichia coli was chemically modified by polyethylene glycol (PEG) of molecular weights 4,500 or 10,000. The neutrophils observed at 32 hours after intravenous injection of the rHuG-CSF modified with PEG (4,500) or PEG (10,000) to mice were, respectively, 2.5 times and 5 times more than that observed after the injection of the unmodified rHuG-CSF. These results show that the covalent attachment of PEG to rHuG-CSF enhanced its pharmacological activity in vivo and that the modification with the larger PEG molecule is more effective to enhance the in vivo activity of rHuG-CSF.

引用

页码：157 / 160

页数：4

共 16 条

[1] ABUCHOWSKI A, 1984, CANCER BIOCHEM BIOPH, V7, P175
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] CHEMICAL MODIFICATION OF RECOMBINANT INTERLEUKIN-2 BY POLYETHYLENE-GLYCOL INCREASES ITS POTENCY IN THE MURINE METH-A SARCOMA MODEL
KATRE, NV
KNAUF, MJ
LAIRD, WJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (06) : 1487 - 1491
[4] KNAUF MJ, 1988, J BIOL CHEM, V263, P15064
[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[6] Maeda H., 1986, PROTEIN TAILORING FO, P353
[7] METCALF D, 1986, BLOOD, V67, P257
[8] MIYATA K, 1988, AGR BIOL CHEM TOKYO, V52, P1575
[9] MOORE RN, 1983, J IMMUNOL, V131, P2374
[10] MOLECULAR-CLONING AND EXPRESSION OF CDNA FOR HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR
NAGATA, S
TSUCHIYA, M
ASANO, S
KAZIRO, Y
YAMAZAKI, T
YAMAMOTO, O
HIRATA, Y
KUBOTA, N
OHEDA, M
NOMURA, H
ONO, M
[J]. NATURE, 1986, 319 (6052) : 415 - 418

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