INCREASED SUSCEPTIBILITY TO TRANSGLUTAMINASE OF EYE LENS PROTEINS EXPOSED TO ACTIVATED OXYGEN SPECIES PRODUCED IN THE GLUCOSE-GLUCOSE OXIDASE REACTION

被引：6

作者：

BROSSA, O ^{[1
]}

SECCIA, M ^{[1
]}

GRAVELA, E ^{[1
]}

机构：

[1] UNIV TURIN,OSPED SAN LUIGI,DIPARTIMENTO SCI CLIN & BIOL,GEN PATHOL SECT,I-10125 TURIN,ITALY

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 11卷 / 4-5期

关键词：

OXYRADICALS; CRYSTALLINS; TRANSGLUTAMINASE; AGING;

D O I：

10.3109/10715769009088919

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to test whether a mild oxidative stress could promote the transglutaminase damaging effect on eye lens proteins, total lens soluble proteins and purified βL-crystallin have been exposed to H2O2: slowly produced by the glucose-glucose oxidase reaction. Soon after the pretreatment, the substrate capacity of the lens proteins for an exogenous transglutaminase has been evaluated. Exposure to the oxidative stress increased the susceptibility of the lens proteins to transglutaminase. When ferrous ions were added to the preincubation medium, in order to convert the H2O2 into the hydroxyl radical, the increase was more evident. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：223 / 229

页数：7

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