INDUCTION OF CYCLOOXYGENASE PROTEIN AND STIMULATION OF PROSTAGLANDIN E(2) RELEASE BY EPIDERMAL GROWTH-FACTOR IN CULTURED GUINEA-PIG GASTRIC MUCOUS CELLS

被引：27

作者：

NAKANO, O ^{[1
]}

SAKAMOTO, C ^{[1
]}

MATSUDA, K ^{[1
]}

KONDA, Y ^{[1
]}

MATOZAKI, T ^{[1
]}

NISHISAKI, H ^{[1
]}

WADA, K ^{[1
]}

SUZUKI, T ^{[1
]}

UCHIDA, T ^{[1
]}

NAGAO, M ^{[1
]}

KASUGA, M ^{[1
]}

机构：

[1] KOBE UNIV, SCH MED, DEPT INTERNAL MED 2, CHUO KU, KOBE 650, JAPAN

来源：

DIGESTIVE DISEASES AND SCIENCES | 1995年 / 40卷 / 08期

关键词：

GASTRIC MUCOSAL CELLS; EPIDERMAL GROWTH FACTOR; PROSTAGLANDIN E(2); CYCLOOXYGENASE ACTIVITY;

D O I：

10.1007/BF02212688

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The present study was undertaken to investigate whether epidermal growth factor (EGF) could stimulate prostaglandin E(2) release, and if so, by what mechanism EGF would exert such an effect in gastric mucosal cells. In cultured guinea pig gastric mucous cells, EGF dose-dependently stimulated prostaglandin E(2) release, with maximal stimulation observed at 10 ng/ml. EGF stimulated an increase in cyclooxygenase activity, which was reduced by protein synthesis inhibitor, actinomycin D, and cycloheximide. EGF also stimulated the enzyme protein synthesis estimated by Western blot analysis, whereas EGF did not stimulate phospholipase A(2) activity. These results suggest that such an effect of EGF on de novo synthesis of cyclooxygenase protein and prostaglandin E(2) release may be involved at least in part in the mechanism of EGF-induced local regulation of gastric mucosal integrity.

引用

页码：1679 / 1686

页数：8

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