CHARACTERISTICS OF AN INULINASE PRODUCED BY BACILLUS-SUBTILIS-430A, A STRAIN ISOLATED FROM THE RHIZOSPHERE OF VERNONIA-HERBACEA (VELL-RUSBY)

被引：39

作者：

VULLO, DL ^{[1
]}

COTO, CE ^{[1
]}

SINERIZ, F ^{[1
]}

机构：

[1] NATL UNIV TUCUMAN,MIRCEN,PROIM,CATEDRA MICROBIOL SUPER,TUCUMAN,ARGENTINA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 08期

关键词：

D O I：

10.1128/AEM.57.8.2392-2394.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus subtilis 430A, isolated from the Vernonia herbacea (Vell Rusby) rhizosphere, produced an exocellular inulinase that fits the requirements for the production of syrups on an industrial scale. The partially purified enzyme, obtained by acetone precipitation, displayed a higher specificity for inulin (K(m), 8 mM) than for sucrose (56 mM) and a total invertase/total inulase ratio of 0.62. In addition, it is stable at an optimal temperature of 45 to 50-degrees-C for at least 7 h and is inhibited by the end product, fructose, at 14 mM.

引用

页码：2392 / 2394

页数：3

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