MOLECULAR-SIZE CHARACTERIZATION OF BACTERIAL CAPSULAR POLYSACCHARIDE VACCINES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：7

作者：

BUSSAT, B ^{[1
]}

SCHULZ, D ^{[1
]}

ARMINJON, F ^{[1
]}

VALENTIN, C ^{[1
]}

ARMAND, J ^{[1
]}

机构：

[1] INST MERIEUX,RES DEPT,1541 AVE MARCEL MERIEUX,F-69280 MARCY LETOILE,FRANCE

来源：

BIOLOGICALS | 1990年 / 18卷 / 02期

关键词：

D O I：

10.1016/1045-1056(90)90021-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial capsular polysaccharides are major virulence factors and some are used as vaccinal antigens. Their molecular size is an important physicochemical criterion which correlates with immunogenicity. This article describes a new application of high performance liquid chromatography (HPLC), based on molecular sieving, for such an evaluation. This HPLC method is rapid, accurate, reproducible, requires only very low amounts of product and presents good correlation with conventional gel permeation chromatography. © 1990.

引用

页码：117 / 121

页数：5

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