PROBING THE PRIMARY QUINONE ENVIRONMENT IN PHOTOSYNTHETIC BACTERIAL REACTION CENTERS BY LIGHT-INDUCED FTIR DIFFERENCE SPECTROSCOPY

被引：77

作者：

BRETON, J

THIBODEAU, DL

BERTHOMIEU, C

MANTELE, W

VERMEGLIO, A

NABEDRYK, E

机构：

[1] CEN CADARACHE,DPVE,F-13108 ST PAUL DURANCE,FRANCE

[2] UNIV FREIBURG,INST BIOPHYS & STRAHLENBIOL,W-7800 FREIBURG,GERMANY

来源：

FEBS LETTERS | 1991年 / 278卷 / 02期

关键词：

FOURIER TRANSFORM INFRARED SPECTROSCOPY; BACTERIAL REACTION CENTER; PHOTOSYNTHESIS; PRIMARY QUINONE; SECONDARY QUINONE;

D O I：

10.1016/0014-5793(91)80129-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photoreduction of the primary electron acceptor, Q(A), has been characterized by light-induced Fourier transform infrared difference spectroscopy for Rb. sphaeroides reaction centers and for Rsp. rubrum and Rp. viridis chromatophores. The samples were treated both with redox compounds, which rapidly reduce the photooxidized primary electron donor P+, and with inhibitors of electron transfer from Q(A)- to the secondary quinone Q(B). This approach yields spectra free from P and P+ contributions which makes possible the study of the microenvironment of Q(A) and Q(A)-.

引用

页码：257 / 260

页数：4

共 20 条

[1]

BAGLEY KA, 1990, CURRENT RES PHOTOSYN, V1, P77

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