EXPRESSION OF THE CHOLECYSTOKININ GENE IN PEDIATRIC TUMORS

被引:13
作者
FRIEDMAN, JM
VITALE, M
MAIMON, J
ISRAEL, MA
HOROWITZ, ME
SCHNEIDER, BS
机构
[1] LONG ISL JEWISH MED CTR, ALBERT EINSTEIN COLL MED, DEPT MED, DIV ENDOCRINOL & METAB, NEW HYDE PK, NY 11042 USA
[2] ROCKEFELLER UNIV, HOWARD HUGHES MED INST, NEW YORK, NY 10021 USA
[3] ROCKEFELLER UNIV, MOLEC BIOL LAB, NEW YORK, NY 10021 USA
[4] NCI, DIV CANC THERAPEUT PEDIAT BRANCH, BETHESDA, MD 20892 USA
[5] UNIV CALIF SAN FRANCISCO, BRAIN TUMOR RES CTR, SAN FRANCISCO, CA 94143 USA
关键词
ECTOPIC HORMONE PRODUCTION; SMALL-ROUND-CELL TUMORS; NEUROEPITHELIOMA; EWING SARCOMA; NEUROPEPTIDES;
D O I
10.1073/pnas.89.13.5819
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have examined a wide range of cultured human tumor cell lines and found that a specific subset of tumors expresses the cholecystokinin (CCK) gene. All neuroepitheliomas (eight) and Ewing sarcoma (eight) cell lines that were tested express CCK RNA. In addition, two of six rhabdomyosarcoma cell lines also express the CCK gene, suggesting that rhabdomyosarcomas are probably heterogenous and that a subset may be similar to Ewing sarcoma and neuroepithelioma. Very few of the positive tumors express completely processed immunoreactive CCK. However, we have used a radioimmunoassay that detects the CCK precursor to demonstrate synthesis of CCK precursor-like peptides by all of the Ewing sarcoma and neuroepithelioma lines that were tested and by the rhabdomyosarcoma cell line that expresses CCK mRNA. These data demonstrate a consistent association of CCK gene expression with a specific group of human neoplasms. The data also add credence to the theory that Ewing sarcoma and neuroepithelioma are derived from the same transformed cell type. Finally, our results suggest that CCK gene expression may serve as a marker to distinguish these tumors, which are considered to be small-round-cell tumors of childhood, from other pediatric tumors.
引用
收藏
页码:5819 / 5823
页数:5
相关论文
共 48 条
[1]   IS THE C-TERMINAL FLANKING PEPTIDE OF RAT CHOLECYSTOKININ DOUBLE SULFATED [J].
ADRIAN, TE ;
DOMIN, J ;
BACARESEHAMILTON, AJ ;
BLOOM, SR .
FEBS LETTERS, 1986, 196 (01) :5-8
[2]  
BIEDLER JL, 1973, CANCER RES, V33, P2643
[3]  
BLOOM ET, 1972, CANCER RES, V32, P960
[4]  
BRODEUR GM, 1977, CANCER-AM CANCER SOC, V40, P2256, DOI 10.1002/1097-0142(197711)40:5<2256::AID-CNCR2820400536>3.0.CO
[5]  
2-1
[6]  
Cavazzana A O, 1988, Prog Clin Biol Res, V271, P487
[7]  
CAVAZZANA AO, 1987, AM J PATHOL, V127, P507
[8]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[9]  
DICKMAN PS, 1982, LAB INVEST, V47, P375
[10]  
Dockray G.J., 1983, P851