PHENYLALANINE AS SUBSTRATE FOR TYROSINE-HYDROXYLASE IN BOVINE ADRENAL CHROMAFFIN CELLS

被引：34

作者：

FUKAMI, MH

HAAVIK, J

FLATMARK, T

机构：

[1] Department of Biochemistry, University of Bergen

来源：

BIOCHEMICAL JOURNAL | 1990年 / 268卷 / 02期

关键词：

D O I：

10.1042/bj2680525

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of bovine chromaffin cells with L-[14C]phenylalanine resulted in label accumulation in catecholamines at about 30% of the rate seen with L-tyrosine as precursor. Studies with purified tyrosine hydroxylase (EC 1.14.16.2) showed that the enzyme catalysed the hydroxylation of L-phenylalanine first to L-p-tyrosine and then to 3,4-dihydroxyphenylalanine (DOPA). No evidence for a significant involvement of an L-m-tyrosine intermediate in DOPA formation was found.

引用

页码：525 / 528

页数：4

共 22 条

[1] ENHANCEMENT OF RAT-BRAIN CATECHOLAMINE SYNTHESIS BY ADMINISTRATION OF SMALL DOSES OF TYROSINE AND EVIDENCE FOR SUBSTRATE-INHIBITION OF TYROSINE-HYDROXYLASE ACTIVITY BY LARGE DOSES OF THE AMINO-ACID
BADAWY, AAB
WILLIAMS, DL
[J]. BIOCHEMICAL JOURNAL, 1982, 206 (01) : 165 - 168
[2] FORMATION OF CATECHOLAMINES FROM PHENYLALANINE IN BRAIN - EFFECTS OF CHLORPROMAZINE AND CATRON
BAGCHI, SP
ZARYCKI, EP
[J]. BIOCHEMICAL PHARMACOLOGY, 1973, 22 (11) : 1353 - 1368
[3] BREAKEFIELD XO, 1974, P NATL ACAD SCI USA, V75, P2530
[4] DIX TA, 1987, BIOCHEMISTRY-US, V26, P3361
[5] SOLUBLE TYROSINE-HYDROXYLASE (TYROSINE 3-MONOOXYGENASE) FROM BOVINE ADRENAL-MEDULLA - LARGE-SCALE PURIFICATION AND PHYSICOCHEMICAL PROPERTIES
HAAVIK, J
ANDERSSON, KK
PETERSSON, L
FLATMARK, T
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 953 (02) : 142 - 156
[6] ISOLATION AND CHARACTERIZATION OF TETRAHYDROPTERIN OXIDATION-PRODUCTS GENERATED IN THE TYROSINE 3-MONOOXYGENASE (TYROSINE-HYDROXYLASE) REACTION
HAAVIK, J
FLATMARK, T
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 168 (01): : 21 - 26
[7] RAPID AND SENSITIVE ASSAY OF TYROSINE 3-MONOOXYGENASE ACTIVITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING THE NATIVE FLUORESCENCE OF DOPA
HAAVIK, J
FLATMARK, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1980, 198 (04): : 511 - 515
[8] HAYCOCK JW, 1982, J BIOL CHEM, V257, P2641
[9] RETROVIRAL TRANSFER OF A HUMAN TYROSINE-HYDROXYLASE CDNA IN VARIOUS CELL-LINES - REGULATED RELEASE OF DOPAMINE IN MOUSE ANTERIOR-PITUITARY ATT-20 CELLS
HORELLOU, P
GUIBERT, B
LEVIEL, V
MALLET, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) : 7233 - 7237
[10] HYDROXYLATION OF PHENYLALANINE BY PURIFIED PREPARATIONS OF ADRENAL AND BRAIN TYROSINE HYDROXYLASE
IKEDA, M
LEVITT, M
UDENFRIE.S
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1965, 18 (04) : 482 - &

← 1 2 3 →