A RAPID METHOD FOR THE DETECTION OF THE ROUS-ASSOCIATED ENDOGENOUS SOLITARY LONG TERMINAL REPEAT, EV15

被引：13

作者：

BENKEL, BF ^{[1
]}

SMITH, EJ ^{[1
]}

机构：

[1] USDA ARS,AVIAN DIS & ONCOL LAB,E LANSING,MI 48823

来源：

POULTRY SCIENCE | 1993年 / 72卷 / 08期

关键词：

ENDOGENOUS SOLITARY LONG TERMINAL REPEAT DETECTION; RAPID NONRADIOACTIVE ASSAY; PROVIRAL FLANKING SEQUENCES; CHICKENS; POLYMERASE CHAIN REACTION-BASED TEST;

D O I：

10.3382/ps.0721601

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A rapid method has been developed for the detection of the solitary long terminal repeat ev15, a member of the avian leukosis virus (ALV) family of endogenous viral elements (ev genes) in chickens. Detection is accomplished by a polymerase chain reaction (PCR) assay that can be performed on purified genomic DNA samples or crude preparations of partially purified whole blood lysates. The test discriminates unambiguously between birds that are homozygous ev15-, homozygous ev15+, or heterozygous ev15-/ev15+. The incorporation of a modified touchdown amplification profile significantly improved the specificity of the PCR assay. Small-scale screening of birds from a variety of chicken breeds has revealed that ev15 is present in populations of both egg-strain birds and broilers.

引用

页码：1601 / 1605

页数：5

共 16 条

[1] ENDOGENOUS VIRAL GENES ARE NON-ESSENTIAL IN THE CHICKEN [J].