PROMOTION OF PARALLEL DNA QUADRUPLEXES BY A YEAST TELOMERE BINDING-PROTEIN - A CIRCULAR-DICHROISM STUDY

被引：203

作者：

GIRALDO, R ^{[1
]}

SUZUKI, M ^{[1
]}

CHAPMAN, L ^{[1
]}

RHODES, D ^{[1
]}

机构：

[1] MRC, MOLEC BIOL LAB, CAMBRIDGE CB2 2QH, ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 16期

关键词：

D O I：

10.1073/pnas.91.16.7658

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Repressor-activator protein 1 (RAP1) has an essential role in the maintenance of yeast telomeres. Yeast telomeric DNA consists of simple repeated G-rich sequences that are bound by RAP1. We have found that RAP1, in addition to its known binding activity for double-stranded DNA, interacts with the G-rich strand containing guanine base (G)-tetrads. We show here using circular dichroism spectroscopy that RAP1 promotes the formation of one particular type of DNA quadruplex, parallel G4-DNA. Furthermore, RAP1 is able to bind to both preformed parallel and antiparallel DNA quadruplexes. These results have implications for the possible use of DNA quadruplexes in telomere-telomere association in vivo.

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页码：7658 / 7662

页数：5

相关论文

共 28 条

[1] NMR-STUDY OF PARALLEL-STRANDED TETRAPLEX FORMATION BY THE HEXADEOXYNUCLEOTIDE D(TG4T) [J].

ABOULELA, F ;

MURCHIE, AIH ;

LILLEY, DMJ .

NATURE, 1992, 360 (6401) :280-282

[2] HAIRPIN AND PARALLEL QUARTET STRUCTURES FOR TELOMERIC SEQUENCES [J].

BALAGURUMOORTHY, P ;

BRAHMACHARI, SK ;

MOHANTY, D ;

BANSAL, M ;

SASISEKHARAN, V .

NUCLEIC ACIDS RESEARCH, 1992, 20 (15) :4061-4067

[3] CHARACTERIZATION OF A G-QUARTET FORMATION REACTION PROMOTED BY THE BETA-SUBUNIT OF THE OXYTRICHA TELOMERE-BINDING PROTEIN [J].

FANG, GW ;

CECH, TR .

BIOCHEMISTRY, 1993, 32 (43) :11646-11657

[4] THE BETA-SUBUNIT OF OXYTRICHA TELOMERE-BINDING PROTEIN PROMOTES G-QUARTET FORMATION BY TELOMERIC DNA [J].

FANG, GW ;

CECH, TR .

CELL, 1993, 74 (05) :875-885

[5] DISTORTION OF THE DNA DOUBLE HELIX BY RAP1 AT SILENCERS AND MULTIPLE TELOMERIC BINDING-SITES [J].

GILSON, E ;

ROBERGE, M ;

GIRALDO, R ;

RHODES, D ;

GASSER, SM .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (02) :293-310

[6] THE YEAST TELOMERE-BINDING PROTEIN RAP1 BINDS TO AND PROMOTES THE FORMATION OF DNA QUADRUPLEXES IN TELOMERIC DNA [J].

GIRALDO, R ;

RHODES, D .

EMBO JOURNAL, 1994, 13 (10) :2411-2420

[7] 4-STRANDED NUCLEIC-ACID STRUCTURES 25 YEARS LATER - FROM GUANOSINE GELS TO TELOMER DNA [J].

GUSCHLBAUER, W ;

CHANTOT, JF ;

THIELE, D .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1990, 8 (03) :491-511

[8] CHARACTERIZATION OF THE DNA-BINDING DOMAIN OF THE YEAST RAP1 PROTEIN [J].

HENRY, YAL ;

CHAMBERS, A ;

TSANG, JSH ;

KINGSMAN, AJ ;

KINGSMAN, SM .

NUCLEIC ACIDS RESEARCH, 1990, 18 (09) :2617-2623

[9] THERMODYNAMICS AND STRUCTURE OF A DNA TETRAPLEX - A SPECTROSCOPIC AND CALORIMETRIC STUDY OF THE TETRAMOLECULAR COMPLEXES OF D(TG3T) AND D(TG3T2G3T) [J].

JIN, RZ ;

GAFFNEY, BL ;

WANG, C ;

JONES, RA ;

BRESLAUER, KJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (18) :8832-8836

[10] CRYSTAL-STRUCTURE OF 4-STRANDED OXYTRICHA TELOMERIC DNA [J].

KANG, C ;

ZHANG, XH ;

RATLIFF, R ;

MOYZIS, R ;

RICH, A .

NATURE, 1992, 356 (6365) :126-131