CALCIUM PROMOTES MEMBRANE ASSOCIATION OF RETICULOCYTE 15-LIPOXYGENASE

被引：32

作者：

WATSON, A ^{[1
]}

DOHERTY, FJ ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,QUEENS MED CTR,SCH MED,DEPT BIOCHEM,NOTTINGHAM NG7 2UH,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1994年 / 298卷

关键词：

D O I：

10.1042/bj2980377

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reticulocyte 15-lipoxygenase (linoleate:oxygen oxido-reductase, EC 1.13.11.12) is implicated in oxidative damage to reticulocyte mitochondria before their elimination by degradation during maturation to the erythrocyte. A proportion of the 15-lipoxygenase sediments with the mitochondrial-rich stromal fraction of density-gradient-fractionated rabbit reticulocytes suggesting a physical association with mitochondria before their elimination. Ca2+ promotes binding of reticulocyte 15-lipoxygenase to isolated rat liver and reticulocyte mitochondria and 15-lipoxygenase-mediated lipid peroxidation of mitochondrial lipids and free linoleic acid. Association of reticulocyte 15-lipoxygenase with isolated mitochondria is not simply a consequence of Ca2+-induced swelling, but implies that Ca2+ mediates translocation of soluble lipoxygenase to mitochondrial membranes. Therefore, Ca2+ may have an important physiological role in the regulation of 15-lipoxygenase-mediated targeting of reticulocyte mitochondria for degradation.

引用

页码：377 / 383

页数：7

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