DIRECT SEQUENCE EVALUATION OF THE MAJOR OUTER-MEMBRANE PROTEIN GENE VARIANT REGIONS OF CHLAMYDIA-TRACHOMATIS SUBTYPES D', I', AND L2'

被引：51

作者：

DEAN, D

PATTON, M

STEPHENS, RS

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143

[2] UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143

[3] UNIV CALIF SAN FRANCISCO,FRANCIS I PROCTOR FDN,SAN FRANCISCO,CA 94143

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 04期

关键词：

D O I：

10.1128/IAI.59.4.1579-1582.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The nucleotide sequences of variable segments (VS) 1, 2, and 4 for the major outer membrane protein gene (omp1) of Chlamydia trachomatis were determined for serologically defined subtypes D', I', and L2'. Asymmetric DNA amplification was used to produce single-stranded DNA for direct sequencing. Amino acid substitutions were detected in VS1, VS2, and VS4 for I', in VS2 for L2', and in VS4 for D'. DNA sequencing of ompl variant regions may be an important method for evaluating the molecular epidemiology of Chlamydia spp.

引用

收藏

页码：1579 / 1582

页数：4

相关论文

共 22 条

[1] MAPPING ANTIGENIC DOMAINS EXPRESSED BY CHLAMYDIA-TRACHOMATIS MAJOR OUTER-MEMBRANE PROTEIN GENES [J].

BAEHR, W ;

ZHANG, YX ;

JOSEPH, T ;

SU, H ;

NANO, FE ;

EVERETT, KDE ;

CALDWELL, HD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) :4000-4004

[2] RESPIRATORY-TRACT COLONIZATION AND A DISTINCTIVE PNEUMONIA SYNDROME IN INFANTS INFECTED WITH CHLAMYDIA-TRACHOMATIS [J].

BEEM, MO ;

SAXON, EM .

NEW ENGLAND JOURNAL OF MEDICINE, 1977, 296 (06) :306-310

[3] EPITOPE MAPPING WITH SOLID-PHASE PEPTIDES - IDENTIFICATION OF TYPE-REACTIVE, SUBSPECIES-REACTIVE, SPECIES-REACTIVE AND GENUS-REACTIVE ANTIBODY-BINDING DOMAINS ON THE MAJOR OUTER-MEMBRANE PROTEIN OF CHLAMYDIA-TRACHOMATIS [J].

CONLAN, JW ;

CLARKE, IN ;

WARD, ME .

MOLECULAR MICROBIOLOGY, 1988, 2 (05) :673-679

[4]

DAWSON CR, 1981, GUIDE TRACHOMA CONTR, P9

[5] TRACHOMA VACCINE STUDIES ON TAIWAN [J].

GRAYSTON, JT ;

WANG, SP ;

WOOLRIDGE, RL .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1962, 98 (01) :352-&

[6] GENERATION OF SINGLE-STRANDED-DNA BY THE POLYMERASE CHAIN-REACTION AND ITS APPLICATION TO DIRECT SEQUENCING OF THE HLA-DQA LOCUS [J].

GYLLENSTEN, UB ;

ERLICH, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7652-7656

[7] CHLAMYDIA-TRACHOMATIS INFANT PNEUMONITIS - COMPARISON WITH MATCHED CONTROLS AND OTHER INFANT PNEUMONITIS [J].

HARRISON, HR ;

ENGLISH, MG ;

LEE, CK ;

ALEXANDER, ER .

NEW ENGLAND JOURNAL OF MEDICINE, 1978, 298 (13) :702-708

[8] NEUTRALIZATION OF CHLAMYDIA-TRACHOMATIS CELL-CULTURE INFECTION BY SEROVAR-SPECIFIC MONOCLONAL-ANTIBODIES [J].

LUCERO, ME ;

KUO, CC .

INFECTION AND IMMUNITY, 1985, 50 (02) :595-597

[9] IMMUNE-RESPONSE OF OWL MONKEYS TO TOPICAL VACCINATION WITH IRRADIATED CHLAMYDIA-TRACHOMATIS [J].

MACDONALD, AB ;

MCCOMB, D ;

HOWARD, L .

JOURNAL OF INFECTIOUS DISEASES, 1984, 149 (03) :439-442

[10] INVITRO NEUTRALIZATION OF CHLAMYDIA-TRACHOMATIS WITH MONOCLONAL-ANTIBODY TO AN EPITOPE ON THE MAJOR OUTER-MEMBRANE PROTEIN [J].

PEELING, R ;

MACLEAN, IW ;

BRUNHAM, RC .

INFECTION AND IMMUNITY, 1984, 46 (02) :484-488