ACCURACY OF THE END-POINT ASSAY FOR VIRUS TITRATION

被引：39

作者：

NIELSEN, LK ^{[1
]}

SMYTH, GK ^{[1
]}

GREENFIELD, PF ^{[1
]}

机构：

[1] UNIV QUEENSLAND,DEPT MATH,ST LUCIA 4072,AUSTRALIA

来源：

CYTOTECHNOLOGY | 1992年 / 8卷 / 03期

关键词：

VIRUS TITRATION; END-POINT ASSAY; VIRUS ASSAY; PLAQUE ASSAY;

D O I：

10.1007/BF02522040

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was found to be inconsistent. A superior estimator based on the maximum likelihood principle was developed. The results are discussed in relation to the choice between the endpoint titration assay and the plaque assay, and an alternative two-stage assay is presented.

引用

页码：231 / 236

页数：6