BETA-ADRENOCEPTOR MEDIATED RESPONSES AND SUBTYPES OF BETA-ADRENOCEPTORS IN CULTURED RAT SERTOLI CELLS

被引:10
作者
EIKVAR, L [1 ]
BJORNERHEIM, R [1 ]
ATTRAMADAL, H [1 ]
HANSSON, V [1 ]
机构
[1] UNIV OSLO,RIKSHOSP,DEPT MED 13,N-0317 OSLO,NORWAY
关键词
D O I
10.1016/0960-0760(93)90155-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane particles from Sertoli cell cultures were examined for subtypes of beta-adrenoceptors with a radioligand binding technique using [I-125]iodocyanopindolol and a beta1-selective antagonist (Sandoz 204 545) or a beta2-selective antagonist (ICI 118 551). Biphasic competition curves and modified Eddie-Hofstee plots revealed a relative distribution of approx. 80% beta1-adrenoceptors and 20% beta2-adrenoceptors. Only 45% of the adrenoceptor mediated stimulation of adenylyl cyclase activity was associated with beta1-adrenoceptors, whereas the remaining 55% was mediated via beta2-adrenoceptors. The subtype selective antagonists inhibited isoproterenol stimulated aromatization of testosterone to estradiol-17beta in a concentration-dependent manner. Complete inhibition of beta1-adrenoceptors resulted in a 45% reduction of estradiol-17beta formation, whereas similar inhibition Of beta2-adrenoceptors resulted in only a 35% reduction. It is concluded that cAMP-dependent effects of beta-adrenergic agonists in Sertoli cells are mediated by activation of both beta1- and beta2-adrenoceptors. The discrepancy between the relative number of beta1- and beta2-adrenoceptors and their relative contribution to cAMP production and aromatization indicates that beta2-adrenoceptors in Sertoli cells are more tightly coupled to the adenylyl cyclase system than beta1-adrenoceptors. Furthermore, complete inhibition of either beta1- or beta2-adrenoceptors by subtype selective antagonists, demonstrates a substantial fraction of spareness between agonist activation of the adenylyl cyclase complex and aromatization.
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页码:85 / 91
页数:7
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