A MONOCLONAL-ANTIBODY WHICH BLOCKS THE FUNCTION OF FACTOR-D OF HUMAN-COMPLEMENT

被引：25

作者：

PASCUAL, M

CATANA, E

SPERTINI, F

MACON, K

VOLANAKIS, JE

SCHIFFERLI, JA

机构：

[1] HOP CANTONAL UNIV,DEPT MED,MED CLIN,CH-1211 GENEVA 4,SWITZERLAND

[2] HOP CANTONAL UNIV,DEPT PATHOL,CH-1211 GENEVA 4,SWITZERLAND

[3] UNIV ALABAMA,DEPT MED,BIRMINGHAM,AL 35294

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 127卷 / 02期

关键词：

Alternative pathway (of complement); Complement inhibitor; Factor D; Monoclonal antibody;

D O I：

10.1016/0022-1759(90)90077-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Factor D is an essential enzyme for activation of complement by the alternative pathways (AP). It has been difficult to obtain mouse monoclonal antibodies (Mabs) which block the function of factor D. We have developed a strategy to obtain Mabs using a double screening procedure of the initial clones. We selected the clone whose supernatant had the lowest level of anti-factor D Ab by ELISA and abolished factor D haemolytic activity. Addition of this Mab to human serum was shown to abolish conversion of C2 cobra venom factor, haemolysis of rabbit erytrocytes, and activation of Ce and C5 by cuprophane dialysis membranes. © 1990.

引用

页码：263 / 269

页数：7

共 22 条

[1] MECHANISMS OF GLOMERULAR INJURY IN IMMUNE-COMPLEX DISEASE [J].