PILI OF AN AEROMONAS-HYDROPHILA STRAIN AS A POSSIBLE COLONIZATION FACTOR

被引：33

作者：

HOKAMA, A

HONMA, Y

NAKASONE, N

机构：

[1] Department of Bacteriology, University of the Ryukyus School of Medicine, Nishihara, Okinawa

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 34卷 / 11期

关键词：

D O I：

10.1111/j.1348-0421.1990.tb01069.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aeromonas hydrophila Ae6 had 2 morphologically distinctive kinds of pili. One appeared rigid and straight with a diameter of 9 nm (R-pili). The other appeared wavy and flexible with a diameter of 7 nm (W-pili). W-pili were very few on the cell as compared with R-pili. In this study, W-pili were purified and characterized. The pili consisted of a subunit protein with a molecular weight of 21 kDa as estimated by SDS-PAGE. There was no immunological cross-reaction between W-pili and other cellular components. The strain Ae6 and its purified W-pili adhered to human and rabbit intestine and agglutinated human and rabbit erythrocytes. Organisms pretreated with the Fab fraction of anti-pilus antibody failed to adhere to the intestine. Pretreatment of intestine with purified W-pili blocked adherence of the organisms to the intestine. These results suggest that the W-pili are the colonization factor of A. hydrophila Ae6.

引用

页码：901 / 915

页数：15

共 38 条

[1] AEROMONAS-HYDROPHILA TYPING SCHEME BASED ON PATTERNS OF AGGLUTINATION WITH ERYTHROCYTES AND YEAST-CELLS [J].