LIPASE KINETICS - HYDROLYSIS OF TRIACETIN BY LIPASE FROM CANDIDA-CYLINDRACEA IN A HOLLOW-FIBER MEMBRANE REACTOR

被引:56
作者
GUIT, RPM
KLOOSTERMAN, M
MEINDERSMA, GW
MAYER, M
MEIJER, EM
机构
[1] DSM-Research, Geleen
关键词
LIPASE KINETICS; CANDIDA-CYLINDRACEA; HYDROLYSIS OF TRIACETIN; HOLLOW-FIBER MEMBRANE REACTOR; IMMOBILIZATION;
D O I
10.1002/bit.260380706
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The aptitude of a hollow-fiber membrane reactor to determine lipase kinetics was investigated using the hydrolysis of triacetin catalyzed by lipase from Candida cylindracea as a model system. The binding of the lipase to the membrane appears not to be very specific (surface adsorption), and probably its conformation is hardly altered by immobilization, resulting in an activity comparable to that of the enzyme in its native form. The reaction kinetics defined on the membrane surface area were found to obey Michaelis-Menten kinetics. The specific activity of the lipase in the membrane reactor was found to be significantly higher than in an emulsion reactor. The activity and stability of the enzyme immobilized on a hydrophilic membrane surface seem not to be influenced significantly by the choice of the membrane material. The hollow-fiber membrane reactor is a suitable tool to assess lipase kinetics in a fast and convenient way.
引用
收藏
页码:727 / 732
页数:6
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