COMPARTMENTALIZATION OF LIPOPOLYSACCHARIDE PRODUCTION CORRELATES WITH CLINICAL PRESENTATION IN MENINGOCOCCAL DISEASE

Lipopolysaccharide (LPS, endotoxin) was quantified in plasma and cerebrospinal fluid (CSF) collected simultaneously from patients with systemic meningococcal disease. High levels (median, 3800 ng/L, range. 750-14,000) were present in plasma and low levels (median, 40 ng/L; range, <25-165) in CSF of patients with fulminant septicemia. Conversely, high levels (median, 2500 ng/L; range, <25-500,000) in CSF and low or undetectable levels (median, <25 ng/L; range, <25-210) in plasma were associated with meningitis without septic shock. Levels of LPS were significantly correlated with protein levels in CSF (r = .50, P = .01) and inversely correlated with the ratio of glucose in CSF to that in blood (r = -.62, P = .0005). LPS level in CSF >800 ng/L was significantly associated with greater-than-or-equal-to 10(9) leukocytes/L, protein levels >0.5 g/L, and a glucose ratio <0.5. Thus, quantification of LPS levels in the plasma and CSF in systemic meningococcal disease is a better predictor of pathophysiologic events than is demonstrating the presence of live bacteria as in conventional culture.