Immuno-electron microscopic demonstration of luminal release of serotonin from enterochromaffin cells of rat embryo

被引：11

作者：

Fujimiya, M ^{[1
]}

Okumiya, K ^{[1
]}

Maeda, T ^{[1
]}

机构：

[1] KOCHI MED SCH, DEPT GERIATR, KOCHI 783, JAPAN

来源：

ACTA HISTOCHEMICA ET CYTOCHEMICA | 1995年 / 28卷 / 06期

关键词：

post-embedding immunoelectron microscopy; serotonin; enterochromaffin cells; rat; embryo;

D O I：

10.1267/ahc.28.555

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The evidence to support the luminal release of serotonin (5HT) from the enterochromaffin cells in rat embryo was demonstrated by immune-electron microscopic study combined with HPLC measurement of 5HT levels in the lumen. A considerable amount of 5HT was detected in the lumen of embryonic gut. To examine the subcellular localization of 5HT in the EC cells, post-embedding immunogold method was used. EC cells in embryonic gut showed a variety of characteristic features as follows. EC cells included secretory granules with high electron-dense cores at basal cytoplasm or both basal and apical cytoplasm. Immunogold particles were densely packed throughout the cytoplasm rather than concentrating over cores of the secretory granules. In the apical cytoplasm of embryonic EC cells, immunogold particles were densely accumulated at the matrix and over the microvilli. The present findings suggest that 5HT is released into the intestinal lumen in a diacrine manner from embryonic EC cells.

引用

页码：555 / 563

页数：9

共 20 条

[1]

Ahlman H., Fluorescence histochemical studies on serotonin in the small intestine and the influence of vagal nerve stimulation, Acta Physiol. Scand., pp. 1-30, (1976)

[2]

Ahlman H., Dahlstrom A., Vagal mechanism controlling serotonin release from the gastrointestinal tract and pyloric motor function, J. Auto. Nerv. Sys., 9, pp. 119-140, (1983)

[3]

Branchek T.A., Gershon M.D., Time course of expression of neuropeptide Y, calcitonin gene-related peptide, and NADPH diaphorase activity in neurons of the developing murine bowel and the appearance of 5-hydroxytryptamine in mucosal enterochromaffin cells, J. Comp. Neurol., 285, pp. 262-273, (1989)

[4]

Bulbring E., Crema A., The release of 5-hydroxytryptamine in relation to pressure exerted on the intestinal mucosa, J. Physiol., 146, pp. 18-28, (1959)

[5]

Cunningham F.G., MacDonald P.C., Gant N.F., Leveno K.J., Gilstrap L.C., Physiology of pregnancy, Williams Obstetrics, (1993)

[6]

Erspamer V., Asero B., Identification of enteramine, the specific hormone of the enterochromaffin cell system, as 5-hydroxytryptamine, Nature, 169, pp. 800-801, (1952)

[7]

Facer P., Bishop A.E., Cole G.A., Aitchison M., Kendall C.H., Aswegen G., Penketh R.J.A., Rodek C.H., McKeever P., Polak J.M., Developmental profile of chromogranin, hormonal peptides, and 5-hydroxytryptamine in gastrointestinal endocrine cells, Gastroenterology, 97, pp. 48-57, (1989)

[8]

Forssmann W.G., Orci L., Ultrastructure and secretory cycle of the gastrin-producing cell, Z. Zellforsch, 101, pp. 419-432, (1969)

[9]

Forssmann W.G., Feurle G.E., Yanaihara N., Helmstaedter V., Glotzer J., Buchler M., Greenberg J., Tischbirek K., Lehy T., Ito S., Lichtenberger L.M., Ultrastructure and immunocytochemistry of gastrin cells (G cells), Biomed. Res., 1, pp. 54-66, (1980)

[10]

Fujimiya M., Kimura H., Effect of lateral hypothalamic area lesions of serotonin-containing epithelial cells in rat duodenum, Brain Res. Bull., 27, pp. 521-528, (1991)

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