THE HELIX-LOOP-HELIX TRANSCRIPTION FACTOR USF (UPSTREAM STIMULATING FACTOR) BINDS TO A REGULATORY SEQUENCE OF THE HUMAN INSULIN GENE ENHANCER

被引：64

作者：

READ, ML ^{[1
]}

CLARK, AR ^{[1
]}

DOCHERTY, K ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,QUEEN ELIZABETH HOSP,DEPT MED,BIRMINGHAM B15 2TH,W MIDLANDS,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1993年 / 295卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj2950233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two important sequence elements, designated insulin enhancer binding site 1 (IEB1) or NIR and IEB2 or FAR, are involved in regulating expression of the rat insulin I gene. These elements bind a helix-loop-helix transcription factor, insulin enhancer factor 1 (IEF1). The IEB1 site is highly conserved among insulin genes but the IEB2 site is not conserved. To investigate the factors binding at the equivalent IEBI and IEB2 sites in the human insulin gene enhancer, electrophoretic mobility shift assays were performed using a variety of cell extracts an probes specific for the homologous IEB1 and IEB2 sites. The results indicate that a factor with similar tissue distribution and binding characteristics to those of IEF1 binds to the IEB1 site in the human insulin gene, but that a separate factor, identified as the adenovirus major late transcription factor [MLTF, or upstream stimulating factor (USF)] binds to the IEB2 site.

引用

页码：233 / 237

页数：5

共 39 条

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