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EXPRESSION OF INSULIN-LIKE GROWTH FACTOR-IA AND FACTOR-IB MESSENGER-RNA IN HUMAN LIVER, HEPATOMA-CELLS, MACROPHAGE-LIKE CELLS AND FIBROBLASTS

被引：27
作者：
NAGAOKA, I
SOMEYA, A
IWABUCHI, K
YAMASHITA, T
机构：
[1] Department of Biochemistry, Juntendo University, School of Medicine, Hongo, Bunkyo-ku, Tokyo
来源：
FEBS LETTERS | 1991年 / 280卷 / 01期
关键词：
INSULIN-LIKE GROWTH FACTOR-I; ALTERNATIVE SPLICING; MESSENGER RNA; POLYMERASE CHAIN REACTION; RNASE PROTECTION ASSAY;
D O I：
10.1016/0014-5793(91)80208-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The human insulin-like growth factor-I (IGF-I) gene codes for two transcripts, IGF-IA and IGF-IB mRNAs, formed by alternative splicing. In this study, the expression of these IGF-I mRNA transcripts was examined using human liver, hepatoma cells, macrophage-like cells and fibroblasts. The reverse transcription-polymerase chain reaction revealed that these cells contained both IGF-IA mRNA (representing exons I, II, III and V) and IGF-IB mRNA (representing exons I, II, III and IV). Interestingly, an RNase protection assay using P-32-labeled IGF-IA and IGF-IB exon-specific cRNA probes demonstrated that IGF-IA mRNA was 10-fold more abundant than IGF-IB mRNA in these cells. However, there was no difference in the stabilities of IGF-IA and IGF-IB mRNAs. These observations indicate that IGF-IA mRNA is more expressed than IGF-IB mRNA in these cells independent of their stabilities.
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页码：79 / 83
页数：5
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