A PINK BACTERIUM AS A REPORTER SYSTEM SIGNALING EXPRESSION OF A RECOMBINANT PROTEIN

被引：8

作者：

KADERBHAI, N ^{[1
]}

GALLAGHER, J ^{[1
]}

HE, MY ^{[1
]}

KADERBHAI, MA ^{[1
]}

机构：

[1] UNIV WALES,DEPT BIOCHEM,ABERYSTWYTH SY23 3DD,WALES

来源：

DNA AND CELL BIOLOGY | 1992年 / 11卷 / 07期

关键词：

D O I：

10.1089/dna.1992.11.567

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a set of expression vectors, pEX-PINK/0-3, for high-level production of (un)fused target proteins. The vectors incorporate a 'pink' reporter element, which signals in vivo the expression status of a target gene. A target sequence is cloned between the lambda-P(L) promoter and the downstream mammalian cytochrome b5 gene. Thermo-induction drives transcription of a dicistronic mRNA from which the target protein and cytochrome b5 are independently and concurrently synthesized. Positive expression is indicated by visual transformation of bacteria from a grey/translucent to a bright pink color derived from tandemly expressed holocytochrome b5. The signal can be monitored in vivo spectrophotometrically.

引用

页码：567 / 577

页数：11

共 42 条

[1]

BALBAS P, 1990, METHOD ENZYMOL, V185, P14

[2]

BECHJENSEN E, 1990, BIOTECHNOL BIOENG, V36, P1

[3] AUTOXIDATION OF SOLUBLE TRYPSIN-CLEAVED MICROSOMAL FERROCYTOCHROME B-5 AND FORMATION OF SUPEROXIDE RADICALS [J].