THE EXPRESSION OF SMALL HEAT-SHOCK PROTEINS IN THE MICROFILARIA OF BRUGIA-PAHANGI AND THEIR POSSIBLE ROLE IN DEVELOPMENT

被引：21

作者：

DEVANEY, E ^{[1
]}

EGAN, A ^{[1
]}

LEWIS, E ^{[1
]}

WARBRICK, EV ^{[1
]}

JECOCK, RM ^{[1
]}

机构：

[1] UNIV LIVERPOOL,LIVERPOOL SCH TROP MED,LIVERPOOL L3 5QA,ENGLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 56卷 / 02期

基金：

英国医学研究理事会;

关键词：

BRUGIA-PAHANGI; MICROFILARIA; SMALL HEAT SHOCK PROTEIN; TEMPERATURE SHIFT; DEVELOPMENT BLOCK;

D O I：

10.1016/0166-6851(92)90170-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Development of the microfilariae of Brugia pahangi in the mammalian host is blocked until uptake by a mosquito vector when the developmental cycle is re-initiated. Comparison of the profile of polypeptides labelled in microfilariae cultured at mammalian temperature (37-degrees-C) or mosquito temperature (28-degrees-C) revealed a complex of low-molecular-weight proteins (18 kDa and 22-24 kDa) synthesized only in microfilariae at 37-degrees-C. The synthesis of these proteins was also induced by transfer of microfilariae to 41-degrees-C (i.e., heat. shock conditions), suggesting that these are heat shock proteins. The expression of the small heat shock proteins in the Brugia life cycle is developmentally regulated, as they are not observed in the mature adult female. Their synthesis is strictly temperature dependent and is repressed upon transfer of the microfilariae to 28-degrees-C.

引用

页码：209 / 217

页数：9

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