SUPERIOR GAS-LIQUID-CHROMATOGRAPHY OF METHYL CHOLANOATE ACETATES ON CYANOPROPYLPHENYLSILOXANE LIQUID-PHASES

被引：43

作者：

YOUSEF, IM

FISHER, MM

MYHER, JJ

KUKSIS, A

机构：

[1] UNIV TORONTO, DEPT PATHOL, TORONTO M5S 1A8, ONTARIO, CANADA

[2] UNIV TORONTO, BANTING & BEST DEPT MED RES, TORONTO M5G 1L6, ONTARIO, CANADA

来源：

ANALYTICAL BIOCHEMISTRY | 1976年 / 75卷 / 02期

关键词：

D O I：

10.1016/0003-2697(76)90108-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The resolution and recovery of mixtures of the methyl ester acetates of synthetic and natural bile acids are excellent on GLC columns prepared with 1-3% OV-225 on 100-200 mesh Gas Chrom Q. The columns are operated isothermally at 250-260.degree. C with He as carrier gas and conventional gas chromatographic equipment. Under these conditions complete separation of the major bile acids of rat and human bile is obtained in 30-60 min. This method of analysis is superior to that based on the trifluoroacetyl or trimethylsilyl derivatives, using OV-225 or other liquid phases.

引用

页码：538 / 544

页数：7

共 14 条

[1] BILE ACIDS .28. GAS CHROMATOGRAPHY OF NEW BILE ACIDS AND THEIR DERIVATIVES [J].

ELLIOTT, WH ;

WALSH, LB ;

MUI, MM ;

THORNE, MA ;

SIEGFRIED, CM .

JOURNAL OF CHROMATOGRAPHY, 1969, 44 (3-4) :452-+

[2]

ENEROTH P, 1969, METHOD ENZYMOL, V15, P237

[3] EFFECT OF DIETARY FAT ON BILE ACID METABOLISM IN MAN [J].

GORDON, BA ;

BEVERIDGE, JMR ;

KUKSIS, A .

CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY, 1964, 42 (06) :897-&

[4] COMPARATIVE STUDIES OF BILE SALTS .9. ISOLATION AND CHEMISTRY OF HYOCHOLIC ACID [J].

HASLEWOOD, GAD .

BIOCHEMICAL JOURNAL, 1956, 62 (04) :637-645

[5]

HEPNER GW, 1974, GASTROENTEROLOGY, V66, P556

[6]

KUKSIS A., 1966, METHOD BIOCHEM ANAL, V14, P325, DOI 10.1002/9780470110324.ch8

[7]

KUKSIS A, 1963, CAN J BIOCHEM PHYS, V41, P1355

[8]

KUKSIS A, 1975, LIPID CHROMATOGRAPHI, V2, P479

[9]

MAKITA M., 1963, ANALYT BIOCHEM, V5, P523, DOI 10.1016/0003-2697(63)90072-1

[10]

MARAI L, 1974, J AM OIL CHEM SOC, V51, pA512

← 1 2 →