INFECTIOUS DNA OF THE HUMAN SPUMARETROVIRUS

被引：60

作者：

RETHWILM, A ^{[1
]}

BAUNACH, G ^{[1
]}

NETZER, KO ^{[1
]}

MAURER, B ^{[1
]}

BORISCH, B ^{[1
]}

TERMEULEN, V ^{[1
]}

机构：

[1] UNIV WURZBURG,INST PATHOL,W-8700 WURZBURG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 04期

关键词：

D O I：

10.1093/nar/18.4.733

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An infectious molecular clone (pHSRV) of the human Spumaretrovirus (HSRV) was constructed using viral DNA and cDNA clones. The infectivity of pHSRV was proven by transfection of cell cultures and subsequent infection of susceptible cultures with cell free transfection derived virus. pHSRV derived virus produced foamy virus typical cytopathic effects in susceptible cultures. Infected cells could be stained specifically with foamy virus antisera by means of indirect immunofluorescence. Radiolmmuno precipitation revealed the presence of characteristic HSRV structural proteins in pHSRV infected cultures. By cotransfection of pHSRV and an indicator plasmid it was found that pHSRV is able to transactivate the viral LTR. Viral transcripts were found to be approximately 200 bases longer in pHSRV infected cultures compared to wildtype infected cultures. This difference is most likely due to an insertion of DNA of non-viral origin in the U3 region of the 3′LTR of the infectious clone. © 1990 Oxford University Press.

引用

页码：733 / 738

页数：6

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[2]

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