CERAMIDE SYNTHESIS ENHANCES TRANSPORT OF GPI-ANCHORED PROTEINS TO THE GOLGI-APPARATUS IN YEAST

被引:183
作者
HORVATH, A
SUTTERLIN, C
MANNINGKRIEG, U
MOVVA, NR
RIEZMAN, H
机构
[1] UNIV BASEL, BIOCTR, CH-4056 BASEL, SWITZERLAND
[2] SANDOZ PHARMA AG, CH-4002 BASEL, SWITZERLAND
关键词
GAS1; GLYCOSYLPHOSPHATIDYLINOSITOL; PROTEIN SORTING; SACCHAROMYCES CEREVISIAE; SECRETION; SPHINGOLIPIDS;
D O I
10.1002/j.1460-2075.1994.tb06678.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Inhibition of ceramide synthesis by a fungal metabolite; myriocin, leads to a rapid and specific reduction in the rate of transport of glycosylphosphatidylinositol (GPI)-anchored proteins to the Golgi apparatus without affecting transport of soluble or transmembrane proteins. Inhibition of ceramide biosynthesis also quickly blocks remodelling of GPI anchors to their ceramide-containing, mild base-resistant forms. These results suggest that the pool of ceramide is rapidly depleted from early points of the secretory pathway and that its presence at these locations enhances transport of GPI-anchored proteins specifically. A mutant that is resistant to myriocin reverses its effect on GPI-anchored protein transport without reversing its effects on ceramide synthesis and remodelling. Two hypotheses are proposed to explain the role of ceramide in the transport of GPI-anchored proteins.
引用
收藏
页码:3687 / 3695
页数:9
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