Free Radical Scavenging and Antioxidant Activities of Silymarin Components

被引：79

作者：

Anthony, Kevin P. ^{[1
]}

Saleh, Mahmoud A. ^{[1
]}

机构：

[1] Texas Southern Univ, Dept Chem, Houston, TX 77004 USA

来源：

ANTIOXIDANTS | 2013年 / 2卷 / 04期

关键词：

Silybum marianum; milk thistle; food supplements; hepatoprotection;

D O I：

10.3390/antiox2040398

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Silymarin is an over the counter food supplement that is sold as a liver enhancement and liver protection preparation. It is a major constituent of the seeds of Silybum marianum which is composed of a mixture of seven major components and several minor compounds. The seven major components: taxifolin, silychristin, silydianin, silybin A, silybin B, iso-silybin A and iso-silybin B were isolated and purified from the crude mixture of silymarin using preparative high performance liquid chromatography to determine which were the most effective for liver protection. Free radical scavenging, hydroxyl radical antioxidant capacity, oxygen radical antioxidant capacity, trolox-equivalent antioxidant capacity and total antioxidant capacity antioxidant activities were determined for each of the individual purified components as well as the crude silymarin mixture. Taxifolin was the most effective component for scavenging free radicals in the DPPH assay with an EC50 of 32 mu M far more effective than all other components which showed EC50 ranging from 115 to 855 mu M. Taxifolin was also found to be the most effective antioxidant in the oxygen radical antioxidant capacity (ORAC) assay with a trolox equivalent of 2.43 and the second most effective in the hydroxyl radical antioxidant capacity (HORAC) assay with a gallic acid equivalent of 0.57. Other antioxidants assays did not show significant differences between samples.

引用

页码：398 / 407

页数：10

共 28 条

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