CLONING OF 2 GENES FROM BACILLUS-CIRCULANS WL-12 WHICH ENCODE 1,3-BETA-GLUCANASE ACTIVITY

被引：19

作者：

FISKE, MJ ^{[1
]}

TOBEYFINCHER, KL ^{[1
]}

FUCHS, RL ^{[1
]}

机构：

[1] MONSANTO CO,DEPT BIOL SCI,ST LOUIS,MO 63198

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

关键词：

D O I：

10.1099/00221287-136-12-2377

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two genes encoding distinct 1,3-beta-glucanases have been cloned from Bacillus circulans and expressed in Escherichia coli. A cosmid library of B. circulans WL-12 DNA was constructed in the broad-host-range cosmid pLAFR1 and screened in E. coli for clones which exhibited 1,3-beta-glucanase activity. Two 1,3-beta-glucanase-positive clones were identified which contained genes encoding two independent 1,3-beta-glucanases as shown by biochemical, physical and molecular analyses. The cosmids, designated pMON5401 (27.1 kb insert) and pMON5402 (24.7 kb insert), encoded 68 kDa and 40 kDa 1,3-beta-glucanases, respectively. Both 1,3-beta-glucanases were purified from their respective E. coli strains, characterized biochemically, and were shown to exhibit lytic activity against purified yeast cell wall preparations.

引用

页码：2377 / 2383

页数：7

共 42 条

[1] DELAY OF DISEASE DEVELOPMENT IN TRANSGENIC PLANTS THAT EXPRESS THE TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE [J].