PARTIAL-PURIFICATION AND CHARACTERIZATION OF OXIDOSQUALENE-LANOSTEROL CYCLASE FROM BAKERS-YEAST

被引：23

作者：

HOSHINO, T ^{[1
]}

WILLIAMS, HJ ^{[1
]}

CHUNG, YS ^{[1
]}

SCOTT, AI ^{[1
]}

机构：

[1] TEXAS AGR EXPTL STN,CTR BIOL NMR,DEPT CHEM,COLLEGE STN,TX 77843

来源：

TETRAHEDRON | 1991年 / 47卷 / 31期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0040-4020(01)86485-1

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Partial (120-fold) purification of oxidosqualene-lanosterol cyclase from yeast is described. The enzyme derived from the microsomal fraction converts 1 mM S-squalene oxide to lanosterol in 5 h and has a pH optimum of 6.2, lower than that (pH 7.2) of the hog-liver cyclase catalyzing the same reaction. Although the yeast cyclase is stimulated by high concentrations of potassium phosphate buffer, high concentrations of potassium or sodium chloride inhibit activity. The concentration range of Triton X-100 for optimal activity is 0.7-1.2%.

引用

页码：5925 / 5932

页数：8

共 12 条

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