AVIDIN PROTEIN-CONJUGATED COLUMN FOR DIRECT INJECTION-ANALYSIS OF DRUG ENANTIOMERS IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：51

作者：

ODA, Y

ASAKAWA, N

ABE, S

YOSHIDA, Y

SATO, T

机构：

[1] Department of Physical and Analytical Chemistry, Tsukuba Research Laboratories, Eisai Co., Ltd., Tsukuba-shi, Ibaraki, 300-26, 1-3

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 572卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80478-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new concept in high-performance liquid chromatography supports is proposed for the direct injection analysis of drug enantiomers in plasma. The new supports are designed with disuccinimidyl suberate as a hydrophobic internal region, and avidin protein as a hydrophilic and bulky surface region. Plasma proteins are excluded by the avidin phase and are eluted immediately from the column, whereas low-molecular-mass analytes can penetrate the surface region and interact with disuccinimidyl suberate. Enantiomers interact differentially with avidin, and are thereby separated. This column was used in reversed-phase high-performance liquid chromatographic analysis to determine ketoprofen enantiomers in plasma by direct injection. The recovery of racemic drug from plasma was almost 100%.

引用

页码：133 / 141

页数：9

共 23 条

[1] DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .3. OPTICAL RESOLUTION OF A SERIES OF N-AROYL D,L-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN COVALENTLY BOUND TO SILICA [J].

ALLENMARK, S ;

BOMGREN, B ;

BOREN, H .

JOURNAL OF CHROMATOGRAPHY, 1983, 264 (01) :63-68

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] SUBUNIT COMPOSITION, FUNCTION, AND SPATIAL ARRANGEMENT IN CA2+-ACTIVATED AND MG2+-ACTIVATED ADENOSINE TRIPHOSPHATASES OF ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM [J].

BRAGG, PD ;

HOU, C .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 167 (01) :311-321

[4] SHIELDED HYDROPHOBIC PHASE - A NEW CONCEPT FOR DIRECT INJECTION-ANALYSIS OF BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

GISCH, DJ ;

HUNTER, BT ;

FEIBUSH, B .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 433 :264-268

[5] INTERNAL SURFACE REVERSED-PHASE SILICA SUPPORTS FOR LIQUID-CHROMATOGRAPHY [J].

HAGESTAM, IH ;

PINKERTON, TC .

ANALYTICAL CHEMISTRY, 1985, 57 (08) :1757-1763

[6] BETA-CYCLODEXTRIN BONDED SILICA FOR DIRECT INJECTION-ANALYSIS OF DRUG ENANTIOMERS IN SERUM BY LIQUID-CHROMATOGRAPHY [J].

HAGINAKA, J ;

WAKAI, J .

ANALYTICAL CHEMISTRY, 1990, 62 (10) :997-1000

[7] INTERNAL-SURFACE REVERSED-PHASE SILICA SUPPORT FOR DIRECT INJECTION DETERMINATION OF DRUGS IN BIOLOGICAL-FLUIDS BY LIQUID-CHROMATOGRAPHY [J].

HAGINAKA, J ;

YASUDA, N ;

WAKAI, J ;

MATSUNAGA, H ;

YASUDA, H ;

KIMURA, Y .

ANALYTICAL CHEMISTRY, 1989, 61 (21) :2445-2448

[8]

HASMESTA DM, 1986, P NATL ACAD SCI USA, V83, P4622

[9]

HEARN MTW, 1982, ADV CHROMATOGR, V20, P1

[10] DIRECT LIQUID-CHROMATOGRAPHIC RESOLUTION OF RACEMIC DRUGS USING ALPHA-1-ACID GLYCOPROTEIN AS THE CHIRAL STATIONARY PHASE [J].

HERMANSSON, J .

JOURNAL OF CHROMATOGRAPHY, 1983, 269 (02) :71-80

← 1 2 3 →