CLONING AND EXPRESSION OF A GENE FOR AN 87-KDA BETA-1,3-GLUCANASE OF BACILLUS-CIRCULANS IAM1165 IN ESCHERICHIA-COLI K-12

被引:6
作者
AONO, R [1 ]
YAMAMOTO, M [1 ]
SATO, M [1 ]
NOGI, Y [1 ]
HORIKOSHI, K [1 ]
机构
[1] RIKEN INST PHYS & CHEM RES, SUPERBUGS PROJECT EXPLORATORY RES ADV TECHNOL, WAKO, SAITAMA 351, JAPAN
关键词
D O I
10.1271/bbb.56.1854
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Partially Sau3AI-digested fragments of chromosomal DNA from Bacillus circulans IAM1165, a high producer of beta-1,3-glucanases able to lyse fungal cell walls, were inserted into a BamHI site of the plasmid vector pHSG399. A gene for the glucanase was cloned in Escherichia coli K-12 by the shotgun method. An 8-kb inserted DNA directed synthesis of an 87-kDa endo-beta-1,3-D-glucanase in E. coli. The beta-glucanase gene was in a 2.6-kb EcoRI-SmaI segment within the insert DNA. The enzyme activity was found mainly in the periplasmic fraction of E. coli carrying the gene.
引用
收藏
页码:1854 / 1858
页数:5
相关论文
共 30 条
[1]   ISOLATION AND PARTIAL CHARACTERIZATION OF AN 87-KILODALTON BETA-1,3-GLUCANASE FROM BACILLUS-CIRCULANS IAM1165 [J].
AONO, R ;
SATO, M ;
YAMAMOTO, M ;
HORIKOSHI, K .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (02) :520-524
[2]  
AONO R, 1989, APPL MICROBIOL BIOT, V31, P397
[4]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[5]   GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES [J].
BLIN, N ;
STAFFORD, DW .
NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2303-2308
[6]   CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].
BOLIVAR, F ;
RODRIGUEZ, RL ;
GREENE, PJ ;
BETLACH, MC ;
HEYNEKER, HL ;
BOYER, HW ;
CROSA, JH ;
FALKOW, S .
GENE, 1977, 2 (02) :95-113
[7]   STRUCTURE OF THE BETA-1,3-1,4-GLUCANASE GENE OF BACILLUS-MACERANS - HOMOLOGIES TO OTHER BETA-GLUCANASES [J].
BORRISS, R ;
BUETTNER, K ;
MAENTSAELAE, P .
MOLECULAR & GENERAL GENETICS, 1990, 222 (2-3) :278-283
[8]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[9]  
DOI K, 1973, AGR BIOL CHEM TOKYO, V37, P1619, DOI 10.1080/00021369.1973.10860880
[10]  
FISKE MJ, 1989, J GEN MICROBIOL, V136, P2377