CLONING AND EXPRESSION OF A GENE FOR AN 87-KDA BETA-1,3-GLUCANASE OF BACILLUS-CIRCULANS IAM1165 IN ESCHERICHIA-COLI K-12

被引：6

作者：

AONO, R ^{[1
]}

YAMAMOTO, M ^{[1
]}

SATO, M ^{[1
]}

NOGI, Y ^{[1
]}

HORIKOSHI, K ^{[1
]}

机构：

[1] RIKEN INST PHYS & CHEM RES, SUPERBUGS PROJECT EXPLORATORY RES ADV TECHNOL, WAKO, SAITAMA 351, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1992年 / 56卷 / 11期

关键词：

D O I：

10.1271/bbb.56.1854

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partially Sau3AI-digested fragments of chromosomal DNA from Bacillus circulans IAM1165, a high producer of beta-1,3-glucanases able to lyse fungal cell walls, were inserted into a BamHI site of the plasmid vector pHSG399. A gene for the glucanase was cloned in Escherichia coli K-12 by the shotgun method. An 8-kb inserted DNA directed synthesis of an 87-kDa endo-beta-1,3-D-glucanase in E. coli. The beta-glucanase gene was in a 2.6-kb EcoRI-SmaI segment within the insert DNA. The enzyme activity was found mainly in the periplasmic fraction of E. coli carrying the gene.

引用

页码：1854 / 1858

页数：5

共 30 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF AN 87-KILODALTON BETA-1,3-GLUCANASE FROM BACILLUS-CIRCULANS IAM1165 [J].