THE ROLE OF PROTEIN-KINASE IN HUMAN SYNOVIAL FIBROBLAST GROWTH

被引：9

作者：

MIGITA, K ^{[1
]}

EGUCHI, K ^{[1
]}

TSUKADA, T ^{[1
]}

KAWABE, Y ^{[1
]}

AOYAGI, T ^{[1
]}

NAGATAKI, S ^{[1
]}

机构：

[1] NATL URESHINO HOSP,DEPT ORTHOPED,URESHINO,SAGA 84303,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1765

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The histological features of rheumatoid arthritis (RA) consist of overgrowth of synovial cells. Several growth factors that cause synovial hyperplasia have been identified in RA synovium. The basic-fibroblast growth factor (b-FGF), representing one of these growth factors, may play an important role in the pathogenesis of RA. We examined the b-FGF mediated intracellular signal pathway involved in synovial cell growth. b-FGF-induced synovial cell growth was inhibited by protein tyrosine kinase (PTK) inhibitors, herbimycin A and genistein, but not by H7 that inhibits protein kinase C (PKC). Stimulation of synovial cells with b-FGF resulted in tyrosine phosphorylation of cellular proteins and MAP kinase activation. Our results also demonstrated that b-FGF-mediated activation of MAP kinase was inhibited by herbimycin A indicating that protein tyrosine kinase may he involved in the activation of MAP kinase in human synovial cells. However. inhibition of b-FGF-mediated MAP kinase activation by PKC downregulation did not occur. (C) 1995 Academic Press, Inc.

引用

页码：1066 / 1075

页数：10

共 29 条

[1] SIGNAL-TRANSDUCTION VIA THE MAP KINASES - PROCEED AT YOUR OWN RSK [J].

BLENIS, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (13) :5889-5892

[2]

BROMLEY M, 1984, ATHRITIS RHEUM, V27, P854

[3]

CHAO TSO, 1994, J BIOL CHEM, V269, P7337

[4] RAS-DEPENDENT ACTIVATION OF MAP KINASE PATHWAY MEDIATED BY G-PROTEIN BETA-GAMMA-SUBUNITS [J].

CRESPO, P ;

XU, NZ ;

SIMONDS, WF ;

GUTKIND, JS .

NATURE, 1994, 369 (6479) :418-420

[5] EXTRACELLULAR SIGNALS AND REVERSIBLE PROTEIN-PHOSPHORYLATION - WHAT TO MEK OF IT ALL [J].

CREWS, CM ;

ERIKSON, RL .

CELL, 1993, 74 (02) :215-217

[6]

DAVIS RJ, 1993, J BIOL CHEM, V268, P14553

[7] INVOLVEMENT OF P21RAS IN ACTIVATION OF EXTRACELLULAR SIGNAL-REGULATED KINASE-2 [J].

DEVRIESSMITS, AMM ;

BURGERING, BMT ;

LEEVERS, SJ ;

MARSHALL, CJ ;

BOS, JL .

NATURE, 1992, 357 (6379) :602-604

[8]

EGUCHI K, 1992, J RHEUMATOL, V19, P1925

[9]

HARISS ED, 1976, ARTHRITIS RHEUM, V19, P68

[10] ACTIVATION OF THE MAP KINASE PATHWAY BY THE PROTEIN-KINASE RAF [J].

HOWE, LR ;

LEEVERS, SJ ;

GOMEZ, N ;

NAKIELNY, S ;

COHEN, P ;

MARSHALL, CJ .

CELL, 1992, 71 (02) :335-342

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