CRYSTALLIZATION OF UDP-N-ACETYLGLUCOSAMINE O-ACYLTRANSFERASE FROM ESCHERICHIA-COLI

被引：10

作者：

PFITZNER, U

RAETZ, CRH

RODERICK, SL

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT BIOCHEM,BRONX,NY 10461

[2] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1995年 / 22卷 / 02期

关键词：

LIPOPOLYSACCHARIDE; LIPID A; ENDOTOXIN; PROTEIN STRUCTURE; ACYLTRANSFERASE; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1002/prot.340220212

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of UDP-N-acetylglucosamine O-acyltransferase (IpxA) from Escherichia coli have been obtained from solutions of sodium/potassium phosphate and dimethylsulfoxide. These crystals belong to the cubic space group P2(1)3 (a = 99.0 Angstrom), diffract X-rays to approximately 2.5 Angstrom resolution and contain one subunit of the enzyme in the asymmetric unit. (C) 1995 Wiley-Liss, Inc.

引用

页码：191 / 192

页数：2

共 12 条

[1]

ANDERSON MS, 1993, J BIOL CHEM, V268, P19858

[2]

ANDERSON MS, 1987, J BIOL CHEM, V262, P5159

[3] 1ST COMMITTED STEP OF LIPID-A BIOSYNTHESIS IN ESCHERICHIA-COLI - SEQUENCE OF THE LPXA GENE [J].