EXPRESSION OF THE MULTIPLE-DRUG RESISTANCE GENE (MDR-1) AND EPITOPE MASKING IN CHRONIC LYMPHATIC-LEUKEMIA

被引：58

作者：

CUMBER, PM ^{[1
]}

JACOBS, A ^{[1
]}

HOY, T ^{[1
]}

FISHER, J ^{[1
]}

WHITTAKER, JA ^{[1
]}

TSURUO, T ^{[1
]}

PADUA, RA ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT APPL MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1990年 / 76卷 / 02期

关键词：

D O I：

10.1111/j.1365-2141.1990.tb07876.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Summary Resistance to cytotoxic agents is a common clinical problem in the treatment of chronic lymphatic leukaemia (CLL). The multidrug resistant (MDR) phenotype characterized by increased levels of a specific cell membrane p‐glycoprotein. confers cross resistance to a wide range of structurally dissimilar antineoplastic drugs. We have studied the expression of this p‐glycoprotein in chronic lymphatic leukaemia measured by immunofluorescence using a monoclonal antibody MRK 16 by flow cytometry. Initial results showed that only 12% of lymphocyte samples from CLL patients showed increased p‐glycoprotein, conflicting with a previous observation that 53% of CLL patients had an increased level of mdr‐1 mRNA. Treatment of the cells with neuraminidase to remove sialic acid residues increased the proportion of patients showing increased p‐glycoprotein to 52%. This suggests that in a subset of CLL patients post translational modification of the protein occurs masking the epitope recognized by MRK 16. Abnormal sialylation patterns associated with malignancy are a well‐recognized phenomenon. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：226 / 230

页数：5

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