INVITRO ACTIVITY OF THE NITROGEN-FIXATION REGULATORY PROTEIN FIXJ FROM RHIZOBIUM-MELILOTI

被引:17
作者
BATUT, J
SANTERO, E
KUSTU, S
机构
[1] INRA,CNRS,BIOL MOLEC RELAT PLANTES MICROORGANISMES LAB,F-31326 CASTANET TOLOSAN,FRANCE
[2] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720
关键词
D O I
10.1128/jb.173.18.5914-5917.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cell extracts of an Escherichia coli strain that overproduces the regulatory protein FIXJ from Rhizobium meliloti promoted transcription of fixK, a known FIXJ-dependent gene, in a coupled transcription-translation assay. Activation by FIXJ was dependent on the sigma-70 holoenzyme form of RNA polymerase.
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页码:5914 / 5917
页数:4
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