ISOLATION AND CHARACTERIZATION OF NATURALLY AND ARTIFICIALLY INDUCED HEPATIC METALLOTHIONEINS FROM 2 SPECIES OF FLATFISH - LIMANDA-LIMANDA AND MICROSTOMUS KITT

被引：14

作者：

DUQUESNE, S

RICHARD, A

机构：

[1] Station Marine, URA CNRS 1363, Université des Sciences et Technologies de Lille, Wimereux, F-62930

来源：

MARINE BIOLOGY | 1994年 / 119卷 / 03期

关键词：

D O I：

10.1007/BF00347543

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

The separation of hepatic metallothioneins of Limanda limanda and Microstomus kitt by gel-filtration chromatography, anion-exchange high-performance liquid chromatography (HPLC) and reversed-phase HPLC is described, Two isoforms of metal-binding proteins were isolated by DEAE HPLC chromatography from fish caught in the field and fish injected intraperitoneally with Cd. The amino acid composition of these proteins further purified by reversed-phase HPLC chromatography revealed the existence of metallothioneins. The route of contamination (natural or artificial) of the fish has no effect on the nature of these proteins and one of the two protein isoforms from each species has similar ionic properties.

引用

页码：461 / 470

页数：10

共 30 条

[1] ISOLATION OF NOVEL METAL-BINDING PROTEINS DISTINCT FROM METALLOTHIONEIN FROM SPOTTED SEA-TROUT (CYNOSCION-NEBULOSUS) AND ATLANTIC CROAKER (MICROPOGONIAS-UNDULATUS) OVARIES [J].