RELEASE OF CALCITONIN GENE-RELATED PEPTIDE-LIKE (CGRP-LI) IMMUNOREACTIVITY FROM RAT ISOLATED SOLEUS MUSCLE BY LOW PH, CAPSAICIN AND POTASSIUM

被引：32

作者：

SANTICIOLI, P

DELBIANCO, E

GEPPETTI, P

MAGGI, CA

机构：

[1] A MENARINI PHARMACEUT,DEPT PHARMACOL,VIA SETTE SANTI 3,I-50131 FLORENCE,ITALY

[2] UNIV FLORENCE,DEPT INTERNAL MED,I-50121 FLORENCE,ITALY

来源：

NEUROSCIENCE LETTERS | 1992年 / 143卷 / 1-2期

关键词：

CGRP; PROTON; RAT SOLEUS MUSCLE; CAPSAICIN-SENSITIVE PRIMARY AFFERENT; RUTHENIUM RED;

D O I：

10.1016/0304-3940(92)90223-T

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Capsaicin (10-mu-M), KCl (80 mM) or superfusion with a low pH medium (pH 5 or 6) produced a significant increase of calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) outflow from the superfused rat isolated soleus muscle. CGRP-LI outflow produced by capsaicin or pH 5 medium was totally abolished in a calcium free medium containing EDTA (1 mM) and the effect of pH 5 medium was prevented by a previous application of capsaicin. Ruthenium red (10-mu-M) produced a marked inhibition of CGRP-LI release produced by capsaicin or pH 5 medium (69 and 84%, resp.), without affecting that evoked by KCl. These findings demonstrate that protons activate capsaicin-sensitive primary afferents in rat skeletal muscle through a Ruthenium red-sensitive mechanism. Proton-induced CGRP-LI release in skeletal muscle could be of relevance during exercise and/or skeletal muscle ischemia.

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页码：19 / 22

页数：4

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