MOBILITY IN PYRUVATE-DEHYDROGENASE COMPLEXES WITH MULTIPLE LIPOLYL DOMAINS

被引：7

作者：

MACHADO, RS ^{[1
]}

GUEST, JR ^{[1
]}

WILLIAMSON, MP ^{[1
]}

机构：

[1] UNIV SHEFFIELD, KREBS INST BIOMOLEC RES, DEPT MOLEC BIOL & BIOTECHNOL,FIRTH COURT, WESTERN BANK, SHEFFIELD S10 2UH, ENGLAND

来源：

FEBS LETTERS | 1993年 / 323卷 / 03期

基金：

英国惠康基金;

关键词：

PYRUVATE DEHYDROGENASE COMPLEX; LIPOATE ACETYLTRANSFERASE; LIPOYL DOMAIN; INTERDOMAIN LINKER PEPTIDE; POLYPEPTIDE MOBILITY; NMR;

D O I：

10.1016/0014-5793(93)81349-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-field NMR studies were carried out with genetically-reconstructed pyruvate dehydrogenase (PDH) complexes of Escherichia coli containing from zero to nine lipoyl domains per lipoate acetyltransferase (E2p) subunit. The only significant differences between the NMR spectra were the increasing intensities of the signals derived from the lipoyl domains and their associated linkers, and the much enhanced signal from the E3-binding domain and its linker in complexes that are devoid of lipoyl domains. The results suggest an explanation for the presence of three lipoyl domains per E2p subunit in the wild-type PDH complex, based on its greater inherent mobility, and potentially more efficient active-site coupling, than any of the other complexes.

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页码：243 / 246

页数：4

相关论文

共 15 条

[1] THE EFFECTS OF DELETION MUTAGENESIS ON THE PYRUVATE-DEHYDROGENASE COMPLEX OF ESCHERICHIA-COLI [J].

ANGIER, SJ ;

MILES, JS ;

SRERE, PA ;

ENGEL, PC ;

GUEST, JR .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1987, 15 (05) :832-833

[2] SUBUNIT STRUCTURE OF DIHYDROLIPOYL TRANSACETYLASE COMPONENT OF PYRUVATE-DEHYDROGENASE COMPLEX FROM ESCHERICHIA-COLI [J].

BLEILE, DM ;

MUNK, P ;

OLIVER, RM ;

REED, LJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4385-4389

[3] GENETIC RECONSTRUCTION AND FUNCTIONAL-ANALYSIS OF THE REPEATING LIPOYL DOMAINS IN THE PYRUVATE-DEHYDROGENASE MULTIENZYME COMPLEX OF ESCHERICHIA-COLI [J].

GUEST, JR ;

LEWIS, HM ;

GRAHAM, LD ;

PACKMAN, LC ;

PERHAM, RN .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 185 (04) :743-754

[4]

GUEST JR, 1989, ANN N Y ACAD SCI, V573, P76

[5] A COMPUTER-MODEL ANALYSIS OF THE ACTIVE-SITE COUPLING MECHANISM IN THE PYRUVATE-DEHYDROGENASE MULTIENZYME COMPLEX OF ESCHERICHIA-COLI [J].

HACKERT, ML ;

OLIVER, RM ;

REED, LJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (10) :2907-2911

[6] CONSTRUCTION AND PROPERTIES OF PYRUVATE-DEHYDROGENASE COMPLEXES WITH UP TO 9 LIPOYL DOMAINS PER LIPOATE ACETYLTRANSFERASE CHAIN [J].

MACHADO, RS ;

CLARK, DP ;

GUEST, JR .

FEMS MICROBIOLOGY LETTERS, 1992, 100 (1-3) :243-248

[7] ATOMIC-STRUCTURE OF THE CUBIC CORE OF THE PYRUVATE-DEHYDROGENASE MULTIENZYME COMPLEX [J].

MATTEVI, A ;

OBMOLOVA, G ;

SCHULZE, E ;

KALK, KH ;

WESTPHAL, AH ;

DEKOK, A ;

HOL, WGJ .

SCIENCE, 1992, 255 (5051) :1544-1550

[8] INVESTIGATION OF THE MECHANISM OF ACTIVE-SITE COUPLING IN THE PYRUVATE-DEHYDROGENASE MULTIENZYME COMPLEX OF ESCHERICHIA-COLI BY PROTEIN ENGINEERING [J].

MILES, JS ;

GUEST, JR ;

RADFORD, SE ;

PERHAM, RN .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 202 (01) :97-106

[9] DOMAINS, MOTIFS, AND LINKERS IN 2-OXO ACID DEHYDROGENASE MULTIENZYME COMPLEXES - A PARADIGM IN THE DESIGN OF A MULTIFUNCTIONAL PROTEIN [J].

PERHAM, RN .

BIOCHEMISTRY, 1991, 30 (35) :8501-8512

[10] SEGMENTAL STRUCTURE AND PROTEIN DOMAINS IN THE PYRUVATE-DEHYDROGENASE MULTIENZYME COMPLEX OF ESCHERICHIA-COLI - GENETIC RECONSTRUCTION INVITRO AND H-1-NMR SPECTROSCOPY [J].

RADFORD, SE ;

LAUE, ED ;

PERHAM, RN ;

MILES, JS ;

GUEST, JR .

BIOCHEMICAL JOURNAL, 1987, 247 (03) :641-649