SYNTHESIS OF GLYCOSYLATED PEPTIDE TEMPLATES CONTAINING 6'-O-PHOSPHORYLATED MANNOSE DISACCHARIDES AND THEIR BINDING TO THE CATION-INDEPENDENT MANNOSE 6-PHOSPHATE RECEPTOR

The multiple-column peptide synthesis of eleven glycopeptides containing two 6'-O-phosphorylated mannose disaccharides linked either alpha(1-->2) or alpha(1-->6) is described. Binding-inhibition studies were performed on the cation-independent mannose 6-phosphate receptor, revealing glycopeptides containing two 6'-O-phosphorylated alpha(1-->2)-linked mannose disaccharides to be potent inhibitors of the receptor binding to a phosphomannan core fragment.