THE PUTATIVE PROMOTER FOR A METACYCLIC VSG GENE IN AFRICAN TRYPANOSOMES

被引：23

作者：

NAGOSHI, YL

ALARCON, CM

DONELSON, JE

机构：

[1] UNIV IOWA,GENET PH D PROGRAM,IOWA CITY,IA 52242

[2] UNIV IOWA,HUMAN NUTR PH D PROGRAM,IOWA CITY,IA 52242

[3] UNIV IOWA,HOWARD HUGHES MED INST,DEPT BIOCHEM,IOWA CITY,IA 52242

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1995年 / 72卷 / 1-2期

关键词：

TRYPANOSOME; PROMOTER; GENE REGULATION; LUCIFERASE; VARIANT SURFACE GLYCOPROTEIN;

D O I：

10.1016/0166-6851(95)00062-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During their metacyclic developmental stage, African trypanosomes are coated with one of 12-15 variant surface glycoproteins (VSGs) that define different metacyclic variant antigen types (MVATs). The MVAT VSG genes are located near telomeres of large chromosomes and are expressed without rearrangement in the metacyclic stage. We have cloned and examined the telomere-linked MVAT5 VSG gene and its upstream expression site associated gene (ESAG I) which are separated by 4.5 kb. Within this 4.5-kb intergenic region is an 87-bp sequence that serves as a strong promoter for a luciferase reporter gene in transient transfection assays. This 87-bp sequence is similar, but not identical, to the promoter for another MVAT VSG gene. UV irradiation experiments were used to detect RNA synthesis from this MVAT5 promoter in bloodstream trypanosomes expressing an unrelated VSG. We propose that this sequence is a specific promoter for the MVAT5 VSG mRNA that occurs in about 10% of the trypanosome population during the metacyclic stage of the parasites' life cycle.

引用

页码：33 / 45

页数：13

共 54 条

[1] Borst, Bernards, Van der Ploeg, Michels, Liu, de Lange, Kooter, The control of variant surface antigen synthesis in trypanosomes, Eur. J. Biochem., 137, pp. 383-389, (1983)
[2] Van der Ploeg, Valerio, de Lange, Bernards, Borst, Grosveld, An analysis of cosmid clones of nuclear DNA from Trypanosoma brucei shows that the genes for the variant surface glycoproteins are clustered in the genome, Nucleic Acids Res., 10, pp. 5905-5923, (1982)
[3] Donelson, Mechanisms of antigenic variation in Borrelia hermsii and African trypanosomes, J. Biol. Chem., 270, pp. 7783-7786, (1995)
[4] Van der Ploeg, Control of antigenic variation in African trypanosomes, New Biol., 3, pp. 324-330, (1991)
[5] Pays, Genetics of antigenic variation in African trypanosomes, Res. Microbiol., 142, pp. 731-735, (1991)
[6] Hajduk, Adler, Bertrand, Fearon, Hager, Hancock, Harris, Le Blanc, Moore, Pollard, Priest, Wood, Molecular biology of African trypanosomes: Development of new strategies to combat an old disease, Am. J. Med. Sci., 303, pp. 258-270, (1992)
[7] Lu, Hall, Gay, Donelson, Point mutations are associated with a gene duplication leading to the bloodstream reexpression of a trypanosome metacyclic VSG, Cell, 72, pp. 397-406, (1993)
[8] Alarcon, Son, Hall, Donelson, A monocistronic transcript for a trypanosome variant surface glycoprotein, Mol. Cell. Biol., 14, pp. 5579-5591, (1994)
[9] Van der Ploeg, Schwartz, Cantor, Borst, Antigenic variation in Trypanosoma brucei analyzed by electrophoretic separation of chromosome-sized DNA molecules, Cell, 37, pp. 77-84, (1984)
[10] Lenardo, Rice-Ficht, Kelly, Esser, Donelson, Characterization of the genes specifying two metacylic variable antigen types in Trypanosoma brucei rhodesiense, Proc. Natl. Acad. Sci. USA, 81, pp. 6642-6646, (1984)

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