NEW ION-EXCHANGERS FOR THE CHROMATOGRAPHY OF BIOPOLYMERS

A group of new ion exchangers for the separation of biopolymers is described in which the ionic groups are exclusively located on linear polymer chains grafted on the support surface. This arrangement markedly reduces the contact between the analyte and dthe matrix, thus suppressing unspecific side activities of the support. Further, this "tentacle-lie" arrangement of the ionic groups allows ionic interactions between the ion exchanger and the analyte which are impossible with standard-type exchangers for steric reasons. These changes in the interaction mode manifest themselves in appreciable differences in the selectivities of the two types of ion exchangers. In addition, the new type of arrangement of the binding groups avoids distorsions of the analyte which may occur on interacting with the conventional type. © 1990.