ISOPRENALINE INHIBITS THROMBOXANE-B2 RELEASE FROM U937 CELLS

被引：1

作者：

BRIGHTLING, CE ^{[1
]}

BAKER, AJ ^{[1
]}

FULLER, RW ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT CLIN PHARMACOL,DU CANE RD,LONDON W12 ONN,ENGLAND

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1993年 / 53卷 / 05期

关键词：

BETA(2)-ADRENOCEPTORS; ZYMOSAN; MONOCYTES; MACROPHAGES;

D O I：

10.1002/jlb.53.5.559

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The U937 monocytic cell line and its differentiated macrophage-like form have been well characterized, illustrating many similarities with their analogous in vivo cells. We examined the release of thromboxane B2 (TXB2) from undifferentiated and differentiated cells after stimulation with opsonized zymosan and investigated whether the release of this mediator could be modified by isoprenaline. After stimulation, the U937 cells released TXB2 in a dose-dependent manner, with the release greater from the differentiated cells. The TXB2 released was inhibited by flurbiprofen (> 10(-8) M; P < .01) and isoprenaline (> 10(-6) M; P < .01), and the inhibition was reversed by propranolol (10(-6) M; P < .02). Thus, it is clear that undifferentiated and differentiated U937 cells release TXB2, which can be inhibited by beta2-adrenoceptor stimulation. These findings illustrate an important functional difference between the in vivo macrophages and differentiated U937 cells because beta2-adrenoceptor stimulation does not inhibit mediator release from macrophages.

引用

页码：559 / 562

页数：4

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