LOCALIZATION OF A SEQUENTIAL B-EPITOPE IN THE VP2 PROTEIN OF HEPATITIS-A VIRUS

被引：4

作者：

KULIK, LN

IVANOV, VS

TCHIKIN, LD

OSTROVSKY, AG

IVANOV, VT

机构：

[1] RUSSIAN ACAD SCI, SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM, MOSCOW 117871, RUSSIA

[2] BYELARUSSIAN ACAD SCI, INST BIOORGAN CHEM, MINSK, BELARUS

来源：

FEBS LETTERS | 1995年 / 367卷 / 01期

关键词：

SYNTHETIC PEPTIDE; ANTIGENIC DETERMINANT; HAV BINDING ANTIBODY; ANTIPEPTIDE ANTIBODY; MONOCLONAL ANTIBODY;

D O I：

10.1016/0014-5793(95)00520-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of synthetic peptides derived from the capsid proteins of hepatitis A virus was used to search for B-epitopes. Peptides from the 115-139 region of the VP1 protein, from the 69-99 region of the VP2 protein and peptide 137-150 from the VP3 protein were found to react with monoclonal and polyclonal anti-HAV antibodies. MAPs based on 64-80 and 66-80 fragments of VP3 were reactive as well. Peptides, their conjugates with protein carriers and MAPs were used for antipeptide antibody production. Only free peptide 69-99 from the VP2 protein caused formation of HAV binding antibodies.

引用

页码：85 / 88

页数：4

共 14 条

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