STRUCTURE-FUNCTION STUDIES OF S-ANTIGEN - USE OF PROTEASES TO REVEAL A DOMINANT UVEITOGENIC SITE

被引：10

作者：

DUA, HS

HOSSAIN, P

BROWN, PAJ

MCKINNON, A

FORRESTER, JV

GREGERSON, DS

DONOSO, LA

机构：

[1] Department of Ophthalmology, University of Aberdeen, Medical School, Aberdeen, AB9 2ZD, Scotland

[2] Pathology, University of Aberdeen, Medical School, Aberdeen, AB9 2ZD, Scotland

[3] Department of Ophthalmology, University of Minnesota, Minneapolis, MN

[4] Retina Service, Wills Eye Hospital, Philadelphia, PA

来源：

AUTOIMMUNITY | 1991年 / 10卷 / 02期

关键词：

AUTOIMMUNITY; MONOCLONAL ANTIBODY; PROTEASES; S-ANTIGEN; TRYPSIN; UVEITIS;

D O I：

10.3109/08916939109004819

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Retinal S-antigen induced experimental autoimmune uvettis (EAU) is a severe, predominantly T-cell mediated inflammatory disease of the uveal tract and retina of the eye. Pretreatment of LEW rats with the monoclonal antibody, MAbS2.4.C5, which defines an epitope in S-antigen, has been shown to effectively inhibit the subsequent induction of EAU with S-antigen. Using synthetic peptides and cyanogen bromide fragments of S-anligen we found the binding site of MAbS2.4.C5 to be located at the carboxy terminus of the molecule corresponding to amino acid positions 375 to 380. Limited Staphylococcus aweus V8 protease digestion yielded several polypeptide fragments including one large 43 kD fragment which retained antibody binding to a variety of both polyclonal and monoclonal antibodies which identify epitopes that span the length of the S-antigen. This treatment, however, completely destroys the MAbS2.4.C5 binding site and dramatically reduces uveitopathogenicity. Limited trypsin and papain digestion, on the other hand, had little effect on pathogenicity or on MAbS2.4.C5 binding to S-antigen or its peptide fragments. These results indicate that the carboxy-terminus of S-antigen plays a predominant role in the pathogenesis of EAU. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：153 / 163

页数：11

共 29 条

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