CARICA-PAPAYA LATEX LIPASE - SN-3 STEREOSELECTIVITY OR SHORT-CHAIN SELECTIVITY - MODEL CHIRAL TRIGLYCERIDES ARE REMOVING THE AMBIGUITY

被引：45

作者：

VILLENEUVE, P ^{[1
]}

PINA, M ^{[1
]}

MONTET, D ^{[1
]}

GRAILLE, J ^{[1
]}

机构：

[1] CIDAD CP,LIPOTECH LAB,F-34032 RENNES,FRANCE

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1995年 / 72卷 / 06期

关键词：

CARICA PAPAYA LATEX; CHIRAL TRIGLYCERIDE; ENZYMATIC HYDROLYSIS; RACEMIC TRIGLYCERIDE; STEREOSPECIFICITY; TYPOSELECTIVITY;

D O I：

10.1007/BF02635668

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Short-chain fatty acids are usually located at position sn-3 in natural triglycerides, particulary in dairy fats. As a result, it is extremely difficult to differentiate between sn-3 stereospecificity and short-chain typoselectivity in many lipases and acyltransferases that perform in this way. This ambiguity can be removed through successive use of a chiral triglyceride with a short fatty acid in position sn-1 and of its racemic in controlled hydrolysis reactions. After checking that the proposed method effectively confirmed the type of activity of control biocatalysts (Candida cylindracea nonspecific lipase and Mucor miehei 1-3 regiospecific lipase), we confirmed that Carica papaya latex has a strict sn-3 stereospecificity.

引用

页码：753 / 755

页数：3

共 7 条

[1]

Nelson J.H., Jensen R.G., Pitas R.E., J. of Dairy Sci., 80, (1977)

[2]

Rogalska E., Cudrey C., Ferrato F., Verger R., Chirality, 5, (1993)

[3]

Jensen R.G., De Jong F.A., Lambert-Davis L.G., Hamosh M., Lipids, 29, (1994)

[4]

Sonnet P.E., Foglia T.A., Baillargeon M.W., J. Am. Oil Chem. Soc., 70, (1993)

[5]

Villeneuve P., Pina M., Montet D., Renard G., Graille J., Chem. Phys. of Lipids, 72, (1994)

[6]

Wolff R.L., Castera-Rossignol F.M., Rev. Franc. des Corps Gras, 34, (1987)

[7]

Tanaka Y., Hirano J., Funada T., Concentration of docosahexaenoic acid in glyceride by hydrolysis of fish oil withcandida cylindracea lipase, Journal of the American Oil Chemists Society, 69, (1992)

← 1 →