THE PROGESTERONE-RECEPTOR STIMULATES CELL-FREE TRANSCRIPTION BY ENHANCING THE FORMATION OF A STABLE PREINITIATION COMPLEX

被引：193

作者：

KLEINHITPASS, L ^{[1
]}

TSAI, SY ^{[1
]}

WEIGEL, NL ^{[1
]}

ALLAN, GF ^{[1
]}

RILEY, D ^{[1
]}

RODRIGUEZ, R ^{[1
]}

SCHRADER, WT ^{[1
]}

TSAI, MJ ^{[1
]}

OMALLEY, BW ^{[1
]}

机构：

[1] BAYLOR UNIV,DEPT CELL BIOL,HOUSTON,TX 77030

来源：

CELL | 1990年 / 60卷 / 02期

关键词：

D O I：

10.1016/0092-8674(90)90740-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly purified chicken progesterone receptor (cPR) is shown to stimulate RNA synthesis directly in an in vitro transcription assay. Stimulation of transcription by cPR requires the presence of progesterone response elements (PREs) in the template and can be specifically inhibited by addition of competitor oligonucleotides containing PREs. Binding of receptor to two PREs is cooperative and leads to a synergistic (27-fold) stimulation of transcription. A purified fusion protein containing the DNA binding domain of cPR linked to yeast ubiquitin was produced in E. coli and also functions in the transcription assay. Using this in vitro transcription system, we demonstrate that hormone-free cPR activated by salt treatment induces transcription of a test gene in a hormone-independent manner. Finally, we present evidence that the progesterone receptor acts by facilitating the formation of a stable preinitiation complex at the target gene promoter and thus augments the initiation of transcription by RNA polymerase II. © 1990.

引用

页码：247 / 257

页数：11

共 63 条

[1] STEROID HORMONE-DEPENDENT INTERACTION OF HUMAN PROGESTERONE-RECEPTOR WITH ITS TARGET ENHANCER ELEMENT [J].