BIOCHEMICAL-COMPOSITION AND METABOLIC-ACTIVITY OF SCRIPPSIELLA-TROCHOIDEA (DINOPHYCEAE) RESTING CYSTS

被引:70
作者
BINDER, BJ [1 ]
ANDERSON, DM [1 ]
机构
[1] WOODS HOLE OCEANOG INST, DEPT BIOL, WOODS HOLE, MA 02543 USA
关键词
cyst; dinoflagellate; dormancy; germination; Scrippsiella;
D O I
10.1111/j.0022-3646.1990.00289.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The composition and metabolic activity of cysts of the marine dinoflagellate Scrippsiella trochoidea (Stein) Loeblich were examined during dormancy, quiescence, and germination. On a per cell basis, newly formed cysts contained an order of magnitude more carbohydrate but significantly less protein and chlorophyll a than did exponentially growing vegetative cells. Loss of lipid and carbohydrate from cysts during the initial dormancy period reflected a respiration rate estimated to be 10% of the respiratory activity in vegetative cells. Among older, quiescent cysts the calculated respiration rate decreased further to approximately 1.5% of the vegetative rate and appeared to proceed largely at the expense of carbohydrate reserves. These estimated rates of respiration were in good agreement with direct measurements of cyst oxygen consumption. The transfer of quiescent cysts to conditions permissive for germination resulted in a rapid increase in respiration rate, as evidenced by carbohydrate loss and O2 consumption. The increased respiratory activity was followed by an increase in protein content and, later, by an increase in chlorophyll a content and photosynthetic capacity. Just prior to germination the P/R ratio became greater than 1, and the estimated chlorophyll‐specific photosynthetic activity reached 75% of the rate in vegetative cells. Complete restoration of photosynthetic and respiratory capacity apparently was not achieved until after excystment. These data confirm the common assumption that dinoflagellate cysts represent true “resting” cells, containing extensive energy reserves and displaying greatly reduced metabolic activity. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:289 / 298
页数:10
相关论文
共 69 条
[1]  
ANDERSON DM, 1980, J PHYCOL, V16, P166
[2]   THE EFFECTS OF DARKNESS AND ANAEROBIOSIS ON DINOFLAGELLATE CYST GERMINATION [J].
ANDERSON, DM ;
TAYLOR, CD ;
ARMBRUST, EV .
LIMNOLOGY AND OCEANOGRAPHY, 1987, 32 (02) :340-351
[3]   POTENTIAL IMPORTANCE OF BENTHIC CYSTS OF GONYAULAX-TAMARENSIS AND G GONYAULAX-EXCAVATA IN INITIATING TOXIC DINOFLAGELLATE BLOOMS [J].
ANDERSON, DM ;
WALL, D .
JOURNAL OF PHYCOLOGY, 1978, 14 (02) :224-234
[4]   ULTRASTRUCTURE AND CYTOCHEMISTRY OF RESTING CELL FORMATION IN AMPHORA-COFFAEFORMIS (BACILLARIOPHYCEAE) [J].
ANDERSON, OR .
JOURNAL OF PHYCOLOGY, 1975, 11 (03) :272-281
[5]  
ANDERSON OR, 1976, KURTZ LIMNOL OCEANOG, V21, P452
[6]   VARIATIONS OF LIPID-COMPOSITION DURING FORMATION OF CYSTS IN GREEN-ALGA PROTOSIPHON-BOTRYOIDES [J].
BERKALOFF, C ;
KADER, JC .
PHYTOCHEMISTRY, 1975, 14 (11) :2353-2355
[7]  
Bewley J.D., 1982, PHYSL BIOCH SEEDS RE, V2
[8]  
Bewley J. D., 1983, PHYSL BIOCH SEEDS RE, VI
[9]  
Bibby B.T., 1972, British phycol J, V7, P85
[10]   GREEN LIGHT-MEDIATED PHOTOMORPHOGENESIS IN A DINOFLAGELLATE RESTING CYST [J].
BINDER, BJ ;
ANDERSON, DM .
NATURE, 1986, 322 (6080) :659-661